Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-05-25
1996-06-18
Warden, Jill
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 4, 435 18, 435 34, 435 38, 435 39, C12Q 170
Patent
active
055276679
ABSTRACT:
Viral contamination in water samples is detected using a medium which includes both a substrate for .beta.-galactosidase and E. coli with elevated levels of intracellular .beta.-galactosidase. The substrate is chosen to undergo a detectable change (e.g., colorimetric, fluorometric, photometric, etc.) upon cleavage by .beta.-galactosidase. A water sample to be tested for viral contamination is added to the medium. If coliphages are present in the water sample, they will infect, multiply within, and subsequently lyse the E. coli host. Lysis of the E. coli will allow the release of the intracellular .beta.-galactosidase into the media, whereupon the enzyme will cleave the substrate for a detectable reaction. In one particular application, a colorimetric reagent that serves as a substrate for .beta.-galactosidase is dissolved or dispersed within an agar medium, and in another particular application, a colorimetric reagent that serves as a substrate for .beta.-galactosidase is dissolved or dispersed within a liquid medium. Color changes which result from lytic cell infection of the E. coli hosts are easily monitored by researchers or laboratory technicians. The presence of coliphages in a water sample is indicative of the presence of harmful human and animal enteric viruses in the water.
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Falkinham Joseph O.
Hagedorn Charles
Ijzerman M. Marian
Ludlow Jan M.
The Center for Innovative Technology
Virginia Polytechnic Institute and State University
Virginia Tech Intellectual Properties Inc.
Warden Jill
LandOfFree
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