Water insoluble biologically active material

Details Water insoluble biologically active material Water insoluble biologically active material

1975-10-01

1977-08-23

Naff, David M.

Textiles: fiber preparation

195 68, 195DIG11, 260112R, C07G 702

Patent

active

040438694

ABSTRACT:
The invention relates to biologically active protein molecules, such as enzymes, attached to water-insoluble solids to render the molecules insoluble. In one aspect the attachment is effected by molecules of diazotized m-diamino-benzene adsorbed on a surface of the solid and chemically linked to the protein molecules. This invention also provides methods for reactivating inactivated biologically active material characterized by a water-insoluble solid with a surface to which has been adsorbed molecules of a diazotized aromatic amine chemically bonded to biologically active protein molecules. One method comprises a) adsorbing additional molecules of a diazotized aromatic diamine on said surface, and b) attaching fresh biologically active protein molecules to said additional molecules of diazotized aromatic diamines. Another method comprises a) treating said material with sodium dithionite to regenerate amine groups, and b) diazotizing said amine groups, and c) attaching thereto biologically active protein molecules.

REFERENCES:
patent: 3167485 (1965-01-01), Katchalski et al.
patent: 3639558 (1972-02-01), Csizmas et al.
Silman et al., Some Water-Insoluble Paper Derivatives Biopolymers, vol. 4, 1966 (pp. 441-448).
The Condensed Chemical Dictionary, 8th Ed., Van Nostrand Reinhold Co., N. Y. 1971, (pp. 10r and 68a).

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