Wash solution, test kit and method for the determination of an i

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435810, 435975, 435 792, 436501, 436510, 436518, 436531, 436536, 436538, 436543, 436547, 436 65, 436808, 436809, 436818, 436824, 436826, 252550, G01N 3353, G01N 3348, G01N 33543, G01N 33536

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050174746

ABSTRACT:
An aqueous wash solution is buffered to a pH of from about 5 to about 9 and contains at least about 0.01 weight percent of a compound comprising a dodecyl sulfate anion and an alkali metal or ammonium cation, such as sodium dodecyl sulfate. This wash solution is particularly useful in a method for the determination of an immunological ligand. Particularly, it is useful for washing the immunological complex formed between the ligand and a receptor molecule therefor. Unreacted materials can be readily separated from the complex by the washing, particularly if the separation is carried out using a filtration membrane in a test device. a test kit for ligand determination comprises the wash solution as well as one or more receptors for the ligand, at least one of which is labeled for detection. This kit is particularly useful for measuring human chorionic gonadotropin (hCG) as an early indicator of pregnancy.

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patent: 4921915 (1990-05-01), Dengler et al.
Qualitiere et al., J. Immun., 119, pp. 1645-1651 (1977).

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