VR-2332 viral nucleotide sequence and methods of use

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 231, 4241991, 4242041, C07H 2104, A61K 3902, A61K 3912

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059986018

ABSTRACT:
A nucleotide sequence is provided for the VR-2332 virus, which is capable of causing Porcine Reproductive and Respiratory Syndrome. The nucleotide sequence includes protein coding regions that are inserted into recombinant vectors for the host expression of viral proteins according to a variety of vaccination techniques. Diagnostic assays utilize fragmentary sequences or oligonucleotides to selectively identify the VR-2332 nucleic acids by hybridization or PCR amplification reactions that distinguish VR-2332 nucleotide sequences from other PRRS-causative viruses which are immunologically distinct from VR-2332.

REFERENCES:
Benfield et al. Characterization of swine infertility and respiratory syndrome(SIRS) virus (isolate ATCC VR-2332). J.Vet.Diagn.Invest. vol. 4:127-133, 1992.
Conzelmann et al. "Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus, a Member of the Arterivirus Group". Virology. 193:329-339, 1993.
Meulenberg et al. "Lelystad Virus, the Causative Agent of Porcine Epidemic Abortion and Respiratory Syndrome(PEARS), Is Related to LDV and EAV". Virology. 192:62-72, 1993.
Attachment A-sequence search.
Shibata et al J. Exp. Med. 167:225-230, 1988.
Suarez et al Arch. Vir. 135:89-99, 1994.
Benfield et al J. Vet. Diagn. Invest. 4:127-133, 1992.
Klupp et al Virology 182:732-741, 1991.
Ellis, Chapter 29 in Vaccines, Polotkin et al Eds., WB Saunders.

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