Vortex stabilized electrophoretic separation apparatus

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041801, G01N 2726

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active

049004212

ABSTRACT:
An apparatus for the performance of efficient, large scale separations, particularly electrophoresis and isoelectric focusing, in free solution is described. Inner and outer cylinders define an annular separation chamber for solutions stabilized against convective flow by Taylor vortices generated during rotation of inner cylinder above threshold frequencies. Separation substrates in solution migrate independently in a potential field established between axially distal anodic and cathodic regions of the annular chamber. Axially defined portions of fluid in the chamber identified to contain concentrations of selected substrates are readily separated from other portions of fluid in the chamber.

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patent: 4683042 (1987-07-01), Scott
patent: 4790942 (1988-12-01), Shmidt
W. Y. Tam, John A. Vastano, Harry L. Swinney, and W. Horsthemke, "Regular and Chaotic Chemical Spatiotemporal Patterns", The American Physical Society vol. 61, No. 19, 7 Nov. 1988 (p. 2163).
Cornelius F. Ivory, William A. Gobie, James B. Beckwith, Robert Hergenrother, Michael Malec, "Electromagnetic Stabilization of Weakly COnducting Fluids", Science, vol. 238, p. 58.
Stellan Hjerten, "Free Zone Electrophoresis", Chromatog. Rev., 9 (1967) 122-219.
Patrick Mattock, Gordon F. Aitchison, Alan R. Thomson, "Velocity Gradient Stabilised, Continuous, Free Flow Electrophoresis. A Review.", Separation and Purificaton Methods, 9(1), 1-68 (1980).
John B. Grutzner, Edward A. Patrick, Perry J. Pellechia, and Marisol Vera, "The Continuously Rotated Cellular Reactor", Journal of the American Chemical Society, 1988, vol. 110, No. 3, p. 726.

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