Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy
Patent
1992-10-06
1994-05-17
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Processes of treating materials by wave energy
435288, 435291, 435810, 435817, 436 94, 436501, 204403, 204412, G01N 2726
Patent
active
053125274
ABSTRACT:
The present invention relates to a voltammetric sequence-selective sensor for target polynucleotide sequences which essentially comprises: an immobilized polynucleotide probe having one end covalently bound onto an amperometric electrode. The immobilized probe includes a target binding region capable of hybridizing to the target polynucleotide sequences forming an immobilized heteroduplex having at least a hybridized region. The sequence-selective sensor of the present invention also comprises means for voltammetrically detecting immobilized heteroduplexes. There is also provided a method for detecting the presence of a target polynucleotide analyte in a physiological sample, which comprises the steps of incubating the prepared physiological sample with the voltammetric sequence-selective sensor of the present invention; voltammetrically detecting immobilized heteroduplexes; and comparing the resulting voltammogram with a control voltammogram.
REFERENCES:
patent: 4882013 (1989-11-01), Turner et al.
patent: 5008182 (1991-04-01), Snimsky et al.
patent: 5079351 (1992-01-01), Sninsky et al.
patent: 5185243 (1993-02-01), Ullman et al.
Mikkelsen Susan R.
Millan Kelly M.
Spurmanis Aleksandrs J.
Bell Bruce F.
Concordia University
Niebling John
Woessner Warren D.
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