Radiant energy – Ionic separation or analysis – With sample supply means
Patent
1998-05-29
2000-08-15
Nguyen, Kiet T.
Radiant energy
Ionic separation or analysis
With sample supply means
H01J 4404
Patent
active
061040282
ABSTRACT:
A sample preparation method is disclosed for volatilization and mass spectrometric analysis of nonvolatile high molecular weight molecules. Photoabsorbing molecules having significant sublimation rates at room temperature under vacuum, and preferably containing hydroxy functionalities, are disclosed for use as matrices in matrix-assisted laser desorption/ionization mass spectrometry. The samples are typically cooled in the mass spectrometer to temperatures significantly below room temperature.
REFERENCES:
patent: 4775619 (1988-10-01), Urdea
patent: 4920264 (1990-04-01), Becker
patent: 4935357 (1990-06-01), Szybalski
patent: 5003059 (1991-03-01), Brennan
patent: 5064754 (1991-11-01), Mills
patent: 5075217 (1991-12-01), Weber
patent: 5118937 (1992-06-01), Hillenkamp et al.
patent: 5135870 (1992-08-01), Williams et al.
patent: 5288644 (1994-02-01), Beavis et al.
patent: 5364759 (1994-11-01), Caskey et al.
patent: 5369004 (1994-11-01), Polymeropoulos et al.
patent: 5378602 (1995-01-01), Polymeropoulos et al.
patent: 5464985 (1995-11-01), Cornish et al.
patent: 5468610 (1995-11-01), Polymeropoulos et al.
patent: 5503980 (1996-04-01), Cantor
patent: 5508169 (1996-04-01), Deugau et al.
patent: 5547835 (1996-08-01), Koster
patent: 5580733 (1996-12-01), Levis et al.
patent: 5582979 (1996-12-01), Weber
patent: 5599666 (1997-02-01), Schumm et al.
patent: 5605798 (1997-02-01), Koster
patent: 5622824 (1997-04-01), Koster
patent: 5625184 (1997-04-01), Vestal et al.
patent: 5627369 (1997-05-01), Vestal et al.
patent: 5691141 (1997-11-01), Koster
patent: 5700642 (1997-12-01), Monforte et al.
patent: 5762876 (1998-06-01), Lincoln et al.
patent: 5854486 (1998-12-01), Dreyfus
patent: 5885775 (1999-03-01), Haff et al.
patent: 5888819 (1999-03-01), Goelet et al.
Abrams, et al., "Comprehensive Detection of Single Base Changes in Human Genomic DNA Using Denaturing Gradient Gel Electrophoresis and a GC Clamp," Genomics, 7:463-475, 1990.
Arnott et al., "Construction and performance of a laser desorption/ionization TOF mass spectrometer system: applications to problems in peptide, protein, and DNA structural analysis," The 40th ASMS Conference on Mass Spectrometry and Allied Topics, May 31-Jun. 5, Washington, D.C., pp. 328-329, 1992.
Bai, Jian, et al., "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Restriction Enzyme-Digested Plalsmid DNA Using an Active Nafion Substrate," Rapid Commun. in Mass Spectrometry, 8:687-691, 1994.
Bai, Jian, et al., "Procedures for Detection of DNA by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using a Modified Nafion Film Substrate," Rapid Commun. in Mass Spectrometry, 9:1172-1176, 1995.
Benner and Jaklevic, "DNA Base-Pair Substitutions Detected in Double-Stranded DNA With Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry," Eur. Mass Spectrum., 479-485, 1995.
Benner, Horn, Katz, Jaklevic, "Identification of Denatured Double-stranded DNA by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry," Rapid Commun. in Mass Spectrom., 9:537-540, 1995.
Bergh, et al., "Complete Sequencing of the p53 Gene Provides Prognostic Information in Breast Cancer Patients, Particularly in Relation to Adjuvant Systemic Therapy and Radiotherapy," Nature Medicine, 1:1029-1034, 1995.
Bevan et al., "The analysis of oligonucleotides and their phosphoramidate analogues by LSIMS mass spectrometry," The 39th ASMS Conference on Mass Spectrometry and Allied Topics, pp. 983-984, 1989.
Chang, et al., "Detection of .increment.F508 Mutation of the Cystic Fibrosis Gene by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry," Rapid Commun. in Mass Spectometry, 9:772-774, 1995.
Edmonds et al., "Electrospray ionization mass spectrometry and tandem mass spectrometry of small oligonucleotides," 37th ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, Miami Beach, FL, pp. 844-845, 1989.
Fang, et al., "Simultaneous Analysis of Mutant and Normal Alleles for Multiple Cystic Fibrosis Mutations by the Ligase Chain Reaction," Human Mutation, 6:144-151, 1995.
Fenn, et al., "Electrospray Ionization for Mass Spectrometry Large Biomolecules," Science, 246:64-71, 1989.
Gimon et al., "Are Proton Transfer Reactions of Excited States Involved in UV Laser Desorption Ionization?," Organic Mass Spectormetry, 27:827-830, 1992.
Hegner, Wagner, Semenza, "Ultralarge Atomically Flat Template-Stripped Au Surfaces for Scanning Probe Microscopy," Surface Science, 291:39-46, 1993.
Hunter et al., "Cryogenic Frozen Solution Matrices for Analysis of DNA by Time-of-Flight Mass Spectrometry," Analytical Chemistry, 69:3608-12, 1997.
Huppert et al., "Laser Studies of Proton Transfer," Advances in Chemical Physics, 47:643-679, 1981.
Jacobson et al., "Applications of mass spectrometry to DNA sequencing,", GATA, 8(8):223-229, 1991.
Jurinke, Christian, et al., "Analysis of Ligase Chain Reaction Products Via Matrix-Assisted Laser Desorption/Ionization Time-of-Flight-Mass Spectrometry," Analytical Biochemistry, 237:174-181, 1996.
Karas and Bahr, "Matrix-assisted laser desorption ionization mass spectrometry," Mass Spectrometry Reviews, 10:335-357, 1991.
Kirpekar, Nordhoff, Kristiansen, Roepstorff, Lezius, Hahner, Karas, Hillenkamp, "Matrix Assisted Laser Desorption/Ionization Mass Spectrometry of Enzymatically Synthesized RNA Up to 150 kDa," Nucleic Acids Research, 22(19):3866-3870, 1994.
Kuimelis and McLaughlin, "Cleavage Properties of an Oligonucleotide Containing a Bridged Internucleotide 5'-phosphorothioate RNA Linkage," Nucleic Acids Research, 23:4753-4760, 1995.
Lee, et al., Comparison on Short Tandem Repeat (STR) Detection Using Silver, Fluorescence and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrophotometry (MALDITOF-MS), Proceedings of the Sixth International Symposium on Human Identification, published by Promega Corp., 1995.
Lee, et al., "DNA Sequencing with Dye-Labeled Terminators and T7 DNA Polymerase: Effect of Dyes and dNTPs on Incorporation of Dye-Terminators and Probability Analysis of Termination Fragments," Nucleic Acids Research, 20(10):2471-2483, 1992.
Liang, Gangning, et al., "The Use of 2-Hydroperoxytetrahydrofuran as a Reagent to Sequence Cytosine and to Probe Non-Watson-Crick DNA Structures," Nucleic Acids Research, 23(4):713-719, 1995.
Limbach et al., "Characterization of oligonucleotides and nucleic acids by mass spectrometry," Current Opinion Biotechnology, 6:96-102, 1995.
Liu, Yan-Hui, et al., "Rapid Screening of Genetic Polymorphisms Using Buccal Cell DNA with Detection by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry," Rapid Commun. in Mass Spectrometry, 9:735-743, 1995.
Liu, Yan-Hui, et al., "Use of a Nitrocellulose Film Substrate in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for DNA Mapping and Screening," Anal. Chem., 67:3482-3490, 1995.
Mag, et al., "Synthesis and Selective Cleavage of an Oligodeoxynucleotide Containing a Bridged Internucleotide 5'-Phosphorothioate Linkage," Nucleic Acids Research, 19(7):1437-1441, 1991.
McNeal et al., "A new method for the analysis of fully protected oligonucleotides by .sup.252 Cf-plasma desorption mass spectrometry. 3. Positive ions," J. Am. Chem. Soc., 104:981-984, 1982.
Mizusawa et al., "Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP," Nucl. Acids. Res., 14(3):1319-1325, 1986.
Mock et al., "Sample immobilization protocols for matrix assisted laser desorption mass spectrometry," The 40th ASMS Conference on Mass Spectrometry and Allied Topics, pp. 1921-1922, 1992.
Musser and Kelley, "Sensitivity enhancement for sttic and continuous flow FAB/MS analysis of nucleotides by quaternary amine surfactants," The 39th ASMS Conference on Mass Spectrometry and Allied Topics, pp. 374-375, 1989.
Nelsen, Dogruel, Williams, "Detection of Human IgM at m/z .about. 1 Mda," Rapid Commun. in Mass Spectrom., 9:7, 1995.
Newman, Nwosu, Williams, Cosstick, Seela, Connolly, "Incorporation of a Complete Set of Deoxyandenosine and Thymidine Analogues Suit
Becker Christopher H.
Hunter Joanna M.
Lin Hua
GeneTrace Systems Inc.
Nguyen Kiet T.
LandOfFree
Volatile matrices for matrix-assisted laser desorption/ionizatio does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Volatile matrices for matrix-assisted laser desorption/ionizatio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Volatile matrices for matrix-assisted laser desorption/ionizatio will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2009294