Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Two or more cell types – per se – in co-culture
Patent
1999-06-08
2000-02-29
Brusca, John S.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Two or more cell types, per se, in co-culture
435455, C12N 508, C12N 510
Patent
active
060308362
ABSTRACT:
The present invention is directed to human mesenchymal stem cells isolated from a tissue specimen, such as marrow cells, and to the method of co-culturing isolated mesenchymal stem cells and/or mesenchymal stem cell-derived adipocytes with hematopoietic progenitor cells such that the hematopoietic stem cells retain their phenotype.
REFERENCES:
Stem Cells 15: 303-313, 1997. Koller, MR et al., Importance of parenchymal:stromal cell ratio for the ex vivo reconstitution of human hematopoiesis.
J. Cell. Physiology 165: 386-397, 1995. Dooley, DC et al., Basic fibroblast growth factor and epidermal growth factor downmodulate the growth of hematopoietic cells in long-term stromal cultures.
Verma, I and Somia, N, Nature 389:239-242 (1997), Oct. 22, 1999.
Report and Recommendations of the Panel to Assess the NIH Investment in Research on Gene Therapy. Orkin, S, and Motulsky, A., (www.nih.gov
ews/panelrep.html) 1995, Oct. 22, 1999.
Mbalaviele Gabriel
Pittenger Mark F.
Thiede Mark A.
Brusca John S.
Lillie Raymond J.
Olstein Elliot M.
Osiris Therapeutics, Inc.
Ousley Andrea
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