Vitro headful packaging system for cloning DNA fragments as larg

Details Vitro headful packaging system for cloning DNA fragments as larg Vitro headful packaging system for cloning DNA fragments as larg

1992-09-29

1995-01-03

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 935 31, C12N 1500, C12N 1566

Patent

active

053786181

ABSTRACT:
This relates to an in vitro headful packaging system for cloning foreign DNA fragments as large as 95 kb. Also of concern is a method of cloning and controlling amplification of DNA fragments as large as 95 kb in a vector containing a multicopy replicon under control of a lac promoter as well as vectors useful for cloning foreign fragments of DNA as large as 95 kb and lysogens to prepare pac-cleavage proficient extract and head-tail proficient extract.

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