Vitro engineered, regenerated urinary tract tissue...

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Implant or insert

Reexamination Certificate

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C424S093700, C435S373000, C435S395000

Reexamination Certificate

active

07122200

ABSTRACT:
A method for providing a urinary tract tissue graft composition includes providing a tissue culture frame and a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in the tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. Smooth muscle and urothelial cells are isolated from a tissue specimen of a subject and cultured, and then seeded upon the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. A tissue culture frame in which such a urinary tract tissue graft may be formed is also disclosed.

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Zhang et al. “Co-culture of bladder smooth muscle and urothelial cells on small intestinal submucosa: Potential applications for tissue engineering technology” J. Urol. (Sep. 2000) 164: 928-935.
Lu et al.; “Muscle-Derived Stem Cells Seeded Into Acellular Scaffolds Develop Calcium-Dependent Contractile Activity That Is Modulated By Nicotinic Receptors”; Urology 61:1285-1291, 2003.
Zhang et al.; “‘Co-Culture’ of Bladder Smooth Muscle And Urothelial Cells On Small Intestinal Submococa (sis): Evaluation Of The Best Culture Method For In Vitro Tissue Engineering Techniques”; Pediatrics Journal Supp: Sep., 1999:807-808.

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