Vitamin D assay

Details Vitamin D assay Vitamin D assay

1995-07-14

1998-10-13

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Biological cellular material tested

436161, 436177, G01N 3348

Patent

active

058210202

ABSTRACT:
The present invention provides a method of assaying a sample of blood or blood components, in particular serum, for the presence of 1,25-dihydroxy-vitamin D comprising removing proteins from the sample, incubating the protein-free sample with a salt of periodic acid, isolating 1,25-dihydroxy-vitamin D from the periodate-treated sample, and determining the concentration of 1,25-dihydroxy-vitamin D.

REFERENCES:
BIOSIS 91:11549; A Rapid Assay For 25 Hydroxyvitamin D And 1, 25 Dihydroxyvitamin D 24-Hydroxylase, Anal Biochem 190(1). 1990. Abstract Biosis.
Brumbaugh et al., "Filter Assay for 1.alpha.,25-Dihydroxyvitamin D.sub.3 -Utilization of the Hormone's Target Tissue Chromatin Receptor," Biochemistry 13(20):4091-4097 (1974).
Coldwell et al., "Gas Chromatography-Mass Spectrometry and the Measurement of Vitamin D Metabolites in Human Serum or Plasma," Steroids 49(1-3):156-196 (1987).

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