Vitamin D 3 -responsive sequences located 5′-upstream...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S320100, C435S325000, C536S023200, C536S024100

Reexamination Certificate

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07465541

ABSTRACT:
A 44-bp region that confers vitamin D3-responsiveness to the human p27Kip1promoter in the human p27Kip1transcriptional regulatory mechanism was revealed. This region also conferred vitamin D3-responsiveness to a heterogenous promoter. Moreover, it was found that vitamin D3enhances the binding between the CCAAT box existing in the region and the NF-Y protein and also the binding between the Sp1 sequence (also existing in the region) and the Sp1 protein, thus inducing human p27Kip1transcription. Also revealed were methods of screening for pharmaceutical agents that regulate the expression of the p27Kip1gene using these molecular mechanisms.

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