Vitamin C from sorbosone

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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C435S189000, C435S190000, C435S191000

Reexamination Certificate

active

07544494

ABSTRACT:
The present invention relates to a process for the production of vitamin C from L sorbosone using an aldehyde dehydrogenase which is isolated fromGluconobacter oxydansDSM 4025 (FERM BP-3812), said enzyme having the following physicochemical properties: (a) molecular weight of 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da); (b) substrate specificity as active on aldehyde compounds; (c) Cofactors are pyrroloquinoline quinone and heme c; (d) Optimum pH between 6.4 and 8.2 for vitamin C production from L-sorbosone; and (e) as inhibitors Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and ethylendiamine tetraacetic acid. The process is performed in the presence of a suitable electron acceptor and the vitamin C isolated from the reaction mixture.

REFERENCES:
patent: 6242233 (2001-06-01), Hoshino et al.
patent: 7341854 (2008-03-01), Hoshino et al.
patent: 2005/0153412 (2005-07-01), Hoshino et al.
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patent: WO 03/089634 (2003-10-01), None
patent: WO 03/104445 (2003-12-01), None
Loewus, M.W. et al., “Conversion ofL-Sorbonone toL-Ascorbic Acid by a NADP-Dependent Dehydrogenase in Bean and Spinach Leaf1,”Plant Physiol, vol. 94, pp. 1492-1495 (1990).

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