Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1982-05-24
1984-08-14
Padgett, Benjamin R.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
536 25, 435 4, 435 14, 435 21, 435 25, 436504, 436544, 436545, 436546, 436505, G01N 3360, G01N 3382, G01N 3350, C08G 7704
Patent
active
044657757
ABSTRACT:
Vitamin B.sub.12 in liquid samples, such as human serum or human plasma, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B.sub.12 derivatives. The labelled derivatives are formed from the (d)-monocarboxylic acid isomer of vitamin B.sub.12, which isomer is free from other monocarboxylic acid isomers (and derivatives thereof) of vitamin B.sub.12, by binding to the (d)-isomer, via the carboxylic group, a compound which is itself a label (e.g. an enzyme) or which comprises a label (e.g. a fluorophore), or to which a label is attached (e.g. a histidine ester to which .sup.125 I is attached). The labelled derivatives of the (d)-monocarboxylic acid of vitamin B.sub.12, free from other isomeric monocarboxylic acids and derivatives, are novel and constitute one aspect of the invention.
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Herron C. J.
Moskowitz M.
Padgett Benjamin R.
Technicon Instruments Corporation
Tedesco S. P.
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