Vitamin B 6 -phosphate phosphatase

Details Vitamin B 6 -phosphate phosphatase Vitamin B 6 -phosphate phosphatase

2006-01-10

2006-01-10

Saucier, Sandra E (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S193000, C435S183000, C435S117000, C435S041000

Reexamination Certificate

active

06984510

ABSTRACT:
A purified vitamin B6-phosphate phosphatase (VB6PP), having the following physico-chemical properties: a) Molecular weight: 29,000±5,000 (consisting of a monomer having a molecular weight of 29,000±5,000); b) Co-factor: Mn2+, Mg2+, Co2+, Sn2+, or Ni2+; c) Substrate specificity: active on pyridoxol 5′-phosphate, pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate; d) Optimum temperature: 30–400° C. at pH 7.5; and e) Optimum pH: 7.0–8.0 is disclosed. Also disclosed are a process for producing VB6PP from a microorganism belonging to the genusSinorhizobiumcapable of producing VB6PP, a process for producing vitamin B6from vitamin B6-phosphate (VB6P) utilizing VB6PP, and a cell-free extract of a microorganism belonging to the genusSinorhizobiumcapable of producing VB6PP.

REFERENCES:
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Costello, R.B., ed. 1991. Webster's College Dictionary, Random House. p. 893.
Domenech, C. 1996. Purification and preliminary characterization of Rhizobium meliloti acid phosphatase. Int. J. Bio-Chromatography 2: 1-8.
Love, S.H. 1956. Synthesis of purine intermediates by a cell-free extract of Escherichia coli. J. Bacteriol. 72: 628-631.
Lucchini, A.E., et al. 1990. Choline derivatives increase two different acid phosphatases in Rhizobium meliloti and Pseudomonas aeruginosa. Arch. Microbiol. 153: 596-599.
Tazoe, M., et al., “Biosynthesis of Vitamin B6Rhizobium,”The Journal of Biological Chemistry, vol. 275 (15) 11300-305 (2000).
Fonda, M., “Purification and Characterization of Vitamin B6-Phosphate Phosphatase from Human Erythrocytes,”The Journal of Biological Chemistryvol. 267 (22) 15978-983 (1992).

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