Visual bioassay for rhizobium competition variants

Details Visual bioassay for rhizobium competition variants Visual bioassay for rhizobium competition variants

1985-12-23

1987-12-15

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, C12Q 104

Patent

active

047133309

ABSTRACT:
A simple, generalized method is disclosed for screening a collection of Rhizobium or Bradyrhizobium strains for variants which, in nodulation, have competative abilities above or below a particular experimentally-set criterion. This method involves mixing a Fix.sup.+ test strain with a Fix.sup.- reference strain, followed by inoculation of a legume plant with the mixture and observation of the inoculated plant's foliage for symptoms of nitrogen sufficiency (green foliage) or nitrogen deficiency (yellow foliage). The method is exemplified by the identification of mutants of both R. fredii and B. japonicum which are phenotypically Comp.sup.- on soybeans (Glycine max).

REFERENCES:
Sylvester-Bradley et al--Chem. Abst., vol. 100, (1984), p. 67287a.
Means et al--Soil Science Soc. Proc., vol. 25, pp. 105-108.
Dowling, D. N. et al., (1985), in Nitrogen Fixation Research Progress, eds.
Evans, H. J. et al., ISBN 90-242-3255-7, p. 141.
Eaglesham, A. R. J. et al., (1982), Appl. Environ. Microbiol., 44:611-618.
Berestetskii, O. A. et al., (1983), Mikrobiologiya, (Moscow), 52:651-657.
Brewin, N. J. et al., (1983), J. Gen. Microbiol. 129:2973-2977.
Diatloff, A. and Brockwell, J., (1974), Austral. J. Exp. Agric. Husb., 16:514-521.

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