Viruses expressing chimeric binding proteins

Details Viruses expressing chimeric binding proteins Viruses expressing chimeric binding proteins

1993-01-26

1995-04-04

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435 697, 4351723, 4352523, 4353201, 530350, 536 234, C07K 1300, C12N 701

Patent

active

054034841

ABSTRACT:
In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.

REFERENCES:
patent: 4304863 (1981-03-01), Collins et al.
patent: 4332897 (1982-06-01), Nakano et al.
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4348477 (1982-09-01), Nakano et al.
patent: 4348478 (1982-09-01), Nakano et al.
patent: 4411994 (1983-09-01), Gilbert et al.
patent: 4508826 (1985-11-01), Foor et al.
patent: 4593002 (1986-06-01), Dulbecco
patent: 4595658 (1986-06-01), Zinder et al.
patent: 4595674 (1986-06-01), Tschesche et al.
patent: 4642334 (1987-04-01), Moore et al.
patent: 4703004 (1987-10-01), Hopp et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4757013 (1988-07-01), Inouye et al.
patent: 4769326 (1988-09-01), Rutter
patent: 4769327 (1988-09-01), Stephens et al.
patent: 4774180 (1988-09-01), Toth et al.
patent: 4797363 (1989-01-01), Teodorescu et al.
patent: 4829052 (1989-05-01), Glover et al.
patent: 4894436 (1990-01-01), Auerswald et al.
patent: 4908773 (1990-03-01), Pantoliano et al.
patent: 5010175 (1991-04-01), Rutter et al.
Science 228:1315-17 (14 Jun. 1985), G. P. Smith Filamentous Fusion Phase: Novel Expression Vectors That Display Cloned Antigens on the Vitrion Surfaces.
J. of Biol. Chem. 261:7115-18 (Jun. 5, 1986), Marks et al Production of Native, Correctly Folded Bovine Pancreatic Trypson Inhibitor Escherichia coli.
Nature 309:810-12 (28 Jun. 1984) Nagai et al Generation of .beta.-globin by sequences spectra proteolysis of a hybrid protein procedure in Escherichia coli.
Fijima et al. 1986 (Mar.) Agric. Biol. Chem 50(3) 589-592.
Fepenci T. 1984 Trends in Biol. Sci. 9:44-48.
Probing the topology of a bacterial membrane protein by genetic insertion of a foreing epitope: expression at the cell surface. The EMBO Journal, Alain Charbit, Jean Claude Boulain, Antoinette Ryter and Maurice Hofnung The EMBO Journal vol. 5, No. 11, pp. 3029-3037,1986.
Presentation of Two Epitopes of the preS2 Region of Hepatitis B Virus on Live Recombinant Bacteria; Alain Charbit, Eliane Sobczak, Marie-Louise Michel, Annie Molla, Pierre Tiollais and Maurice Hofnung; J. Immunology, Vo. 139, 1658-1664, No. 5 Sep. 1, 1987.
Immunogenicity and Epitope Mapping of Foreign Sequences via Genetically Engineered Filamentous Phage; Vidal F. de la Cruz, Altaf A. Lal and Thomas F. McCutchan; The Journal of Biological Chemistry vol. 263, No. 9, Issue of Mar. 25, pp. 431804322. 1988.
In Vitro Genertic Constructions Devised to Express Given Antigenic Determinants at the Surface of Gram-negative Bacteria, Bernadette Bouges-Bocquet, Jean-Luc Guesdon, Christian Marchal and Maurice Hofnung; Unite de Programmation Moleculaire et Toxicologie Genetique CNRSLA 271, INSERM U.163, Instit Pasteur, 75015 Paris, France.
Subcellular localization of a PhoE-LacZ fusion protein in E. coli by protease accessibility experiments reveals an inner-membrane-spanning form of the protein; Jan Tommassen and Toon de Kroon; FEB 05071, vol. 221 No. 2, 226-230, Sep. 1987.
Uses of lac Fusions for the Study of Biological Problems; Thomas J. Silhavy and Jonathan R. Beckwith; Microbiological Reviews, Dec. 1985, pp. 398-418, vol. 49, No. 4--American Society for Microbiology.
Sequence Information within the lam.beta. Gene is Required for Proper Routing of the Bacteriophage .lambda.Receptor Protein go the Outer Membrane of Escherichia coli K-12; Michael N. Hall, Maxime Schwartz and Thomas J. Silhavy; J. Med. Biol (1982) 156, 93-112.
Selection for Mutants Altered in the Expression or Export of Outer Membrane Porin OmpF; Erica J. Sodergren, Jane Davidson, Ronald K. Taylor and Thomas J. Silhavy; J. of Bacteriology, Jun. 1985, pp. 1047-1053.
Affinity-Chromatographic Studies Based on the Binding-Specificity of the Lambda Receptor of Escherichia coli, T. Ferenci; Ann. Microbiol. (Inst. Pasteur) 1982, 133 A, 167-169.
Directed Evolution of the Lambda Receptor of Escherichia coli through Affinity Chromatographic Selection; Thomas Ferenci and Kin-Sang Lee J. Mol. Biol. (1982) 160, 431-444.
Isolation, by Affinity Chromatography, of Mutant Escherichia coli Cells with Novel Regulation of lam.beta. Expression; Thomas Ferenci and Kin-Sang Lee J. of Bacteriology, May 1983, pp. 984-987, vol. 154, No. 2.
Affinity Engineering of Maltoporin; Variants with Enhanced Affinity for Particular Ligands: Ann Clune, Kin-Sang Lee and Thomas Ferenci Biochemical and Biophysical Research Communications, vol. 121, No. 1, 1984 pp. 34-40.
Sequence determinants in the lab.beta. gene of Escherichia coli influencing the binding and pore selectivity of maltoporin; Hans-Georg Heine, Joanna Kyngdon and Thomas Ferenci; Gene, 53 (1987) 287-192.
Cloning of random-sequence oligodeoxynucleotides; Arnold R. Oliphant, Alexander L. Nussbaum and Kevin Struhl; Gene. 44 (1986) 177-183.
The Use of Random-Sequence Oligonucleotides for Determining Consensus Sequences; Arnold R. Oliphant and Kevin Struhl: Methods in Enzymology, vol. 155, pp. 568-582 (1987).
Combinatorial Cassette Mutagenesis as a Probe of the Informational Content of Protein Sequences; John F. Reidhaar-Olson and Robert T. Sauer; Research Articles, Science, vol. 241, 1 Jul. 1988, pp. 53-57.
Mutagenesis of the Arc Repressor Using Synthetic Primers with Random Nucleotide Substitutions; Andrew K. Vershon, Karen Blackmer and Robert T. Sauer; Protein Engineering, Applications in Science, Medicine and Industry; 1986, pp. 243-257.
Isolation and Analysis of Arc Repressor Mutants: Evidence for an Unusual Mechanism of DNA Binding; Andrew K. Vershon, James U. Bowie, Theresa M. Karplus and Robert T. Sauer; Proteins: Structure, Function, and Genetics 1:302-311 (1986).
Bacteriophage .lambda.cro mutations: Effects on activity and intracellular degradation; Andrew A. Pakula, Vincent B. Young and Robert T. Sauer; Proc. Natl. Acad. Sci, USA, vol. 83, pp. 8829-8833, Dec. 1986.
Phage Lambda Repressor Revertants--Amino Acid Substitutions that Restore Activity to Mutant Proteins; Michael H. Hecht and Robert T. Sauer J. Med. Biol. (1985) 186, 53-63.
Lambda Repressor Mutations That Increase the Affinity and Specificity of Operator Binding; Hillary C. M. Nelson and Robert T. Sauer; Cell, vol. 42, 549-588, Sep. 1985.
Interaction of Mutant .lambda.Repressors with Operator and Non-Operator DNA; Hillary C. M. Nelson and Robert T. Sauer; J. Med. Biol. (1986) 192, 27-38.
Amino Acid Replacements that Compensate for a Large Polypeptide Deletion in an Enzyme; Carvin Ho, Maria Jasin and Paul Schimmel; Science, vol. 229, pp. 389-393; 26 Jul. 1985.
Mutagenesis by random linker insertion into the lam.beta. gene of Escherichia coli K12; Jean Claude Boulain, Alina Charbit and Maurice Hofnung; Med. Gen. Genes (1986) 205: 339-348.
Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering: James A. Wells, Brian C. Cunningham Thomas P. Graycar, and David A. Estell; Proc. Natl. Acad. Sci. USA, vol. 84, pp. 5167-5171, Aug. 1987.
Mutants of Bovine Pancreatic Trypsin Inhibitor Lacking Cysteines 14 and 38 Can Fold Properly; Cara Berman Marks, Hossein Naderi, Phyllis Anne Kosen, Irwin D. Kuntz and Steph

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