Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1991-09-03
1994-04-19
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
536 236, 536 2372, 4351723, 800DIG42, 935 10, 935 35, 935 67, 935 64, A01H 500, C12N 1529, C12N 1540, C12N 1500
Patent
active
053047303
ABSTRACT:
A DNA sequence encoding a potato leafroll virus coat protein having at least one internal translation initiation codon in a different reading frame than the native PLRV coat protein DNA sequence altered to a non-initiator codon is provided. Two translation initiation sites at the start of a 17kd open reading frame in a different reading frame than the native PLRV coat protein DNA sequence are preferably altered A stronger stop codon is also provided in the modified PLRV DNA sequence. The modified DNA sequence having the internal translation initiation codons at the start of the 17kd open reading frame altered to non-initiator codons can be used in a gene to transform plants of the Solanaceae family to obtain transgenic plants resistant to PLRV. A synthetic modified potato leafroll virus DNA sequence is also provided which has the changes in the translation initiation sites and is further made to be more "plant-like". Multiple copies of the modified or synthetic modified PLRV coat protein DNA sequence driven by different promoters have also been transformed into potato with advantageous results. The modified potato leafroll virus DNA sequences of this invention are particularly useful in obtaining transgenic potato plants resistant to infection by potato leafroll virus
REFERENCES:
patent: 4970168 (1990-11-01), Tumer
Kawchuk, L. M. et al. 1990 Molec. Plant. Microb. Interact. vol. 3 pp. 301-307.
Tacke, E. et al. 1990, J. Gen. Virol. vol. 71 pp. 2265-2272.
Powell, P. A. et al. 1990 Virology vol. 175 pp. 124-130.
Sanger, M. et al. 1990 Plant Molec. Biol vol. 14 pp. 433-443.
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Burpee Gardens catalog 1986, W. Atlee Burpee Co., Warminster, Pa. p. 143.
Hemenway Cynthia L.
Lawson Edgar C.
Tumer Nilgun E.
Weiss James D.
Fox David T.
Hoerner, Jr. Dennis R.
Monsanto Company
Mosher Mary E.
Shear Richard H.
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