Virus resistant plants

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 536 231, 536 2372, 536 241, 800205, 800DIG40, 800DIG42, 800DIG43, 800DIG44, C12N 1500, A01H 104, C07H 1700

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active

055039995

ABSTRACT:
An isolated DNA sequence which codes for a potyvirus replicase gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a replicase gene in plants is also disclosed. Transgenic potato plants and tubers containing the replicase gene are also disclosed.

REFERENCES:
patent: 4970168 (1990-11-01), Tumer
Carr et al. (1992) Molecular Plant Microbe Interactions 5(5):397-404. Resistance to tobacco mosaic virus induced by the 54-kDa gene sequence requires expression of the 54--kDa protein.
Donson et al. (1993) Molecular Plant Microbe Interactions 6(5):635-642. Broad resistance to tobamoviruses is mediated by a modified tobacco mosaic virus replicase transgene.
McFarlane and Davies (1992) Proc. Natl. Acad. Sci. USA 89:5829-5833. Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.
Taschner et al. (1991) Virology 181:445-450. Replication of an incomplete alfalfa mosaic virus genome in plants transformed with viral replicase genes.
Longstaff et al. (1993) EMBO J. 12(2):379-386. Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase.
Anderson et al. (1992) Proc. Natl. Acad. Sci. USA 89:8759-8763. A defective replicase gene induces resistance to cucumber mosaic virus in transgenic tobacco plants.
Day et al. (1991) Proc. Natl. Acad. Sci. USA 88:6721-6725. Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus.
Braun and Hemenway (1992) The Plant Cell 4:735-744. Expression of amino-terminal portions or full-length viral replicase genes in transgenic plants confers resistance to potato virus X infection.
Audy et al (1994) Mol. Plant -Microbe Int. 7(1): 15-21.
Robaglia et al. (1989) J. Gen. Virol 70: 935-947.
Kaniewski et al. (1990) Bio Technology 8: 750-753.
Golemoski et al. (1990) Proc. Natl Acad Sci 87: 6311-6315.
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