Virus induced gene silencing in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule

Reexamination Certificate

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C800S280000

Reexamination Certificate

active

07012172

ABSTRACT:
The present invention discloses methods for interfering with expression of the genes in plant cells by using replicating recombinant viral vectors. A host plant is infected at one or more locations with a recombinant viral vector. The vector is both an initiator and a target of the RNA-triggered gene silencing in plant cells. The vector upon infection is capable of directing self-replication and producing a transcription product of a nucleic acid segment. The transcription product interferes with the expression of a specific gene in plant cells.

REFERENCES:
Kiyosue et al., Plant J., 2000, vol. 23, pp. 807-815.
Thomas et al., Plant J., 2001, vol. 25, pp. 417-425.
Angell et al., EMBO J., 1997, vol. 16, pp. 3675-3684.

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