Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1987-05-12
1990-03-20
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 29, 435 33, 435 34, 4351723, 435320, 436 63, 935 76, 935 27, 935 29, 935 70, G01N 33569, C12N 1500
Patent
active
049101323
ABSTRACT:
A virus-free assay to identify agents that interfere with the life cycle of HIV which comprises: (1) expressing in mammalian cells in the presence or absence of one or more of said agents, HIV gp120, the gene product of HIV tat, the gene product of HIV art/trs, all under the control of an HIV LTR promoter, and a control protein, not under the control of the HIV LTR promoter, such that infectious HIV particles are not produced; (2) quantitating the amount of the gp120 produced relative to the amount of control protein produced; and (3) comparing the relative amount of the gp120 produced in the presence of said one or more agents to the relative amount of the gp120 produced in the absence of said one or more agents. A plasmid capable of expressing the gene product of HIV art/trs in mammalian cells such that infectious HIV particles are not produced, which includes: (1) a DNA fragment comprising an HIV LTR promoter, and (2) and HIV cDNA restriction fragment carrying the art/trs gene but not the ATG start codon of the first coding exon for tat.
REFERENCES:
patent: 4704357 (1987-11-01), Mitsuya et al.
Mitsuya et al., "Rapid in Vitro Systems for Assessing Activity of Agents Against HTLV-III/LAV" in AIDS: Modern Concepts and Therapeutic Challenges, S. Broder, ed., Marcel Dekker Inc., N.Y., 1987, pp. 303-333.
Sodroski et al., "A Second Post-Transcriptional trans-Activator Gene Required for HTLV-III Replication", Nature, 321 (1986) 412-417.
Feinberg et al., "HTLV-III Expression and Production Involve Complex Regulation at the Levels of Splicing and Translation of Viral RNA", Cell, 46 (1986) 807-817.
Fisher et al., "The trans-Activator Gene of HTLV-III is Essential for Virus Replication", Nature, 320 (1986) 367-371.
Dayton et al., "The Trans-Activator Gene of the Human T Cell Lymphotropic Virus Type III is Required for Replication", Cell, 44 (1986) 941-947.
Meusing et al., "Regulation of mRNA Accumulation by a Human Immunodeficiency Virus Trans-Activator Protein", Cell, 48 (1987) 691-701.
Knight et al., "Expression of the Art/Trs Protein of HIV and Study of Its Role in Viral Envelope Synthesis", Science, 236 (1987) 837-840.
Goh et al., Identification of a Protein Encoded by the trans Activator Gene tatIII of Human T-Cell Lymphotropic Retrovirus Type III, J. Virol, 59 (1986) 181-184.
Ghrayeb John
Knight David M.
Centocor
Nucker Christine M.
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