Virulent phages to detect Listeria monocytogenes in...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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C424S093600

Reexamination Certificate

active

08034552

ABSTRACT:
The present invention relates to virulent (lytic)Listeria monocytogenesphage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and/or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and/or shipped, in order to reduceListeria monocytogenescontamination. P100 can also be used in the present invention to identifyListeria monocytogenesbacteria present on (or within) foodstuffs, as well as thoseListeria monocytogenesbacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected withListeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected withListeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and/or with other antimicrobial agents to reduce or eliminateListeria.

REFERENCES:
patent: 5006347 (1991-04-01), Day et al.
patent: 43 26 617 (1994-06-01), None
patent: 2 253 859 (1992-09-01), None
Roy et al., “Biological inactivation of adhering Listeria monocytogenes by listeriaphages and a quaternary ammonium compound”, Applied and Environmental Microbiology, vol. 59, No. 9, 1993, pp. 2914-2917.
Gaeng et al., “Gene cloning and expression and secretion of Listeria monocytogenes bacteriophage-lytic enzymes in Lactococcus lactis”, Applied and Environmental Microbiology, vol. 66, No. 7, Jul. 2000, pp. 2951-2958.
Loessner et al., “Construction of luciferase reporter bacteriophage A511::luxAB for rapid and sensitive detection of viable Listeria cells”, Applied and Environmental Microbiology, vol. 62, No. 4, Apr. 1, 1996, pp. 1133-1140.
Loessner M.J. & Busse M. “Bacteriophage typing of Listeria species”, Applied and Environmental Microbiology, Jun. 1990, 56(6): 1912-1918, entire document.

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