Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1984-02-15
1987-11-03
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 7, 4351721, 2504612, 436546, C12Q 170, G01N 5300, G01N 33533, G01N 2164, C12N 1500
Patent
active
047043547
ABSTRACT:
A cytofluorograph is used to quantify poxvirus concentration. Virions producing a known cell bound antigen are added to a cell culture. The antigen is fluorescently labeled and the infected cells are counted cytofluorographically. Certain mutated virions do not produce the labeled antigen and are not counted. Alternately, the virion infected cell culture is stained with a phenanthridium dye. Only infected cells absorb the dye. Dead cells are counted with a cytofluorograph indicating virion concentration. The two tests combined discriminate between teratogens and carcinogens. Toxins which are teratogens reduce the number of infective virus, whereas carcinogens not only reduce the virus number, but increase the number of mutants.
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Foley Shawn P.
Naff David M.
University of Cincinnati
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