Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Reexamination Certificate
2007-05-29
2007-05-29
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
C435S235100, C435S325000, C435S369000
Reexamination Certificate
active
10424985
ABSTRACT:
The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.
REFERENCES:
patent: 5403484 (1995-04-01), Ladner et al.
patent: 5658779 (1997-08-01), Krupey et al.
patent: 5731187 (1998-03-01), Fanget et al.
patent: 5948441 (1999-09-01), Lenk et al.
patent: 6194191 (2001-02-01), Zhang et al.
patent: 6726907 (2004-04-01), Zhang et al.
patent: 6808916 (2004-10-01), Coffey et al.
patent: 2002/0168764 (2002-11-01), Coffey et al.
patent: 2004/0152183 (2004-08-01), O'Riordan et al.
patent: 63044532 (1988-02-01), None
patent: WO 97/08298 (1997-03-01), None
patent: WO 98/22588 (1998-05-01), None
patent: WO 99/08692 (1999-02-01), None
patent: WO99/08692 (1999-02-01), None
Vellekamp et al. Hum. Gene Therapy. Oct. 10, 2001; 12:1923-36.
Nibert et al. J. Virology. 1996; 70(10):7295-7300.
Liu et al., Investigative Ophthalmology and Visual Science 2006, 47(6):2438-2444.
Nooteboom et al., Clinical and Experimental Immunology 2006, 144:362-369.
Hartwig et al., Transfusion Medicine 2005, 15:107-113.
U.S. Appl. No. 20020037576, published Mar. 28, 2002.
Berry et al., Biotechnology and Bioengineering, “Production of Reovirus Type-1 and Type-3 from Vero Cells Grown on Solid and Macroporous Microcarriers”,Biotechnology and Bioengineering62: 12-19 (1999).
Bos, J.L., “Ras Oncogenes in Human Cancer: A Review”,Canc. Res.49(17): 4682-4689 (1989).
Chandran and Nibert, “Protease cleavage of reovirus capsid protein mu1 and mu1C blocked by alkyl sulfate detergents, yielding a new type of infectious subvirion particle”,J. of Virology72(1):467-75 (1998).
Coffey, M.C., et al., “Reovirus therapy of tumors with activated Ras pathway”,Science282: 1332-1334 (1998).
Drastini, Y. et al., “Comparison of eight different procedures for harvesting avian reoviruses grown in Vero cells”,J. Virological Methods39: 269-278 (1992).
Duncan et al., “Conformational and functional analysis of the C-terminal globular head of the reovirus cell attachment protein”,Virology182(2):810-9 (1991).
Mah et al., “The N-terminal quarter of reovirus cell attachment protein sigma 1 possesses intrinsic virion-anchoring function”,Virology179(1):95-103 (1990).
McRae, M.A. and Joklik, W.K., “The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3”,Virology,89:578-593 (1979).
Nibert et al., “Reovirus and their replication”, in Fields et al.,Fundamental Virology, 3rd Edition,Lippincott-Raven (1996).
Smith, R.E., et al., “Polypeptide components of virions, top component and cores of reovirus type 3”,Virology,39:791-800 (1969).
Strong, J.E. and P.W. Lee, “The v-erbV oncogene confers enhanced cellular susceptibility to reovirus infection”,J. Virol.70: 612-616 (1996).
Strong, J.E., et al., “Evidence that the Epidermal Growth Factor Receptor on Host Cells Confers Reovirus Infection Efficiency”,Virology197(1): 405-411 (1993).
Strong, J.E., et al., “The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus”,EMBO J.17: 3351-3362 (1998).
Taber et al., “The selection of virus-resistant Chinese hamster ovary cells”,Cell8: 529-533 (1976).
Turner and Duncan, “Site directed mutagenesis of the C-Terminal portion of reovirus protein sigmal: evidence for a conformation-dependent receptor binding domain”,Virology186(1):219-27 (1992).
Guzo David
Oncolytics Biotech Inc.
LandOfFree
Viral purification methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Viral purification methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Viral purification methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3737471