Viral production process

Details Viral production process Viral production process

1998-05-04

1999-11-30

Stucker, Jeffrey

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

435 41, 435174, 435177, 4352351, 435239, 435325, 435366, 435369, 435395, 4352891, 435948, C12N 701

Patent

active

059941340

ABSTRACT:
The present invention is directed to a method of producing recombinant viral vectors at high titers incorporating a variety of important advancements over the art. The method of the present invention incorporates multiple features which provide enhanced production of viruses, particularly those viruses encoding exogenous transgenes. The specifically illustrated method describes a method for the high titer serum-free media production of recombinant replication defective adenoviruses containing an exogenous transgene. The invention provides methods of preparing microcarriers, methods for seeding bioreactors at high cell density, increasing the infectivity of the producer cells to the virus, methods to increase product yield through synchronization of the cell cycle of the producer cells, and methods to minimize the deleterious effects of exogenous transgenes. The invention further provides producer cells prepared by the process of the invention. The invention further provides viruses produced by the process.

REFERENCES:
patent: 5219752 (1993-06-01), Takazawa et al.
patent: 5494811 (1996-02-01), Scott et al.

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