Viral-mediated gene transfer system

Details Viral-mediated gene transfer system Viral-mediated gene transfer system

1994-02-10

1997-09-30

Guzo, David

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 936, 4351723, 4353201, 435368, A61K 4800, C12N 1586

Patent

active

056723440

ABSTRACT:
A recombinant neurotropic HSV-1 vector carrying the gene for human HPRT under the direction of the viral TK promoter expresses a high level of human enzyme in host neuronal cells, yet has reduced cytopathicity. The virulence of the HSV-1 vector is further reduced by rendering it replication-defective through UV-irradiation or the use of a replication-defective HSV-1 deletion mutant in its construction. The recombinant vector is also provided with a non-vital site of initiation of DNA synthesis, which permits the HPRT gene to replicate independently of the viral genome, thus providing means for amplification of the gene in the host. The recombinant HSV-1 vector's high level of expression of HPRT coupled with decreased virulence allows treatment of HPRT deficieny by direct infection of the host organism.

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