Viral lysis assay

Details Viral lysis assay Viral lysis assay

1984-05-21

1988-03-15

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435235, 435748, 436501, 436519, 436520, 436522, 436829, C12Q 170, C12N 700, G01N 33554, G01N 33555

Patent

active

047313242

ABSTRACT:
A viral lysis immunoassay system and method. The system includes hemolytic particles carrying non-viral, anti-analyte molecules, lysable target cells which are devoid of surface molecules capable of binding to endogenous viral surface molecules, and foreign binding molecules added to the target cells. The binding molecules, which may be either analyte molecules or analyte-related molecules attached to the target cell surfaces, function to bind the particles to the cells, to initiate cell lysis and the release of encapsulated reporter molecules from the cells. The analyte to be assayed may be one adapted to bridge the virus particles to analyte-related molecules carried on the cell surfaces, or one which competes with target-cell molecules for binding to the particle anti-analyte molecules.

REFERENCES:
patent: 4342826 (1982-08-01), Cole
patent: 4483929 (1984-11-01), Szoka
Sinha, D. et al., Biochem. Biophys. Res. Commun., 90, No. 2: 554-560, (1979).
Tomasi, M. et al., FEBS Letters, 143, No. 2: 252-256, (1982).
Heath, T. et al., (1983), Exp. Cell Res., 149: 163-175.
Bankert, R. et al., (1979), Journ. of Immunol., 123, No. 6: 2466-2473.
Sekiguchi, K. et al., (1978), PNAS 75, No. 4: 1740-1744.
Oku, N. et al., (1982), Virology, 116: 419-427.
Huang, A. et al., (1980), Journ. of Biol. Chem., 255, No. 17: 8015-8018.

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