Viral capsid assembly intermediates and methods of production

Details Viral capsid assembly intermediates and methods of production Viral capsid assembly intermediates and methods of production

2002-09-13

2009-12-29

Parkin, J S (Department: 1648)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S006120, C435S007100, C435S069100

Reexamination Certificate

active

07638269

ABSTRACT:
A cell-free method for translation and assembly of viral capsid and capsid intermediates is disclosed. Also disclosed are novel capsid assembly intermediates and novel host proteins which bind to such assembly intermediates. The invention also includes a screening method for compounds that alter viral capsid assembly, and a method of treating viral infection using compounds which inhibit the capsid assembly pathway.

REFERENCES:
patent: 5324637 (1994-06-01), Thompson et al.
Morikawa et al. Complete inhibition of human immunodeficiency virus Gag myristoylation is necessary for inhibition of particle budding. Journal of Biological Chemistry (Feb. 2, 1996) vol. 271, No. 5, pp. 2868-2873.
Sakalian et al. Synthesis and assembly of retrovirus Gag precursors into immature capsids in vitro. Journal of Virology (Jun. 1996) vol. 70, No. 6, pp. 3706-3715.
Lee et al. Inhibition of wild-type HIV-1 virus production by a matrix deficient Gag mutant. Virology (Apr. 1995) vol. 208, No. 2, pp. 808-811.
Ou et al. Preferred translation of human hepatitis B virus polymerase from core protein- but not from precore protein-specific transcript. Journal of Virology (1990) vol. 64, No. 9, pp. 4578-4581.
Santolini et al. Biosynthesis and biochemical properties of the hepatitis C virus core protein. Journal of Virology (1994) vol. 68, No. 6, pp. 3631-3641.
Plamenberg A.C In vitro synthesis and assembly of Picornaviral capsid intermediate structures. Journal of Virology (1982) vol. 44, pp. 900-906.
Weinheimer et al. Autoproteolysis of Herpes Simplex virus type 1 protease release an active catalytic domain found in intermediate capsid particles. Journal of Virology (1993) vol. 67, pp. 5813-5822.
Cao et al. Protein conformer selection by ligand binding observed with crystallography, Protein Science, 1998, vol. 7, pp. 72-78.
Bisbal et al.,J. Biol. Chem. 270 (22): 13308-133 17 (1995).
Carmichael,Nature418:379-380 (2002).
Erickson and Blobel,Methods Enzymol96:38-50 (1983).
Goncavales et al.,J. Biol. Chem. 277: 3206-3205 (2002).
Hegde et al.Molecular Cell2:85-89 (1998).
Merrick, W.C.,Methods Enzymol. 101:606-615 (1983).
Morris et al.,Nature Biotechnology19:1173-1176 (2001).
Novina et al.,Nature Medicine8:681-686 (2002).
Platt et al.PNAS91:4594-4598 (1994).
Rose et al.,Nucleic Acids Research26:1628-1635 (1998).
Royer, M., et al.,Virology184:417-422 (1991).
Rutkowski et al.PNAS98:7823-7828 (2001).
Spearman et. al.,J. Virology70 (11): 8187-8194 (1996).
Spearman, P. et al.,J. Virol. 68:3232-3242 (1994).
Weldon, Jr., et al.,J Virol72: 3098-106 (1998).
Willison et al.,Cell57:621-632 (1989).
Wills et al.,J. Virol65:3804-3812 (1991).
Degar et al. “Inactivation of the Human Immunodeficiency Virus by Hypericin: Evidence for Photochemical Alterations of p24 and a Block in Uncoating”AIDS Research&Human Retroviruses8(11):1929-1936 (1992).
Fäcke et al. “A Large Deletion in the Matrix Domain of the Human Immunodeficiency VirusgagGene Redirects Virus Particle Assembly from the Plasma Membrane to the Endoplasmic Reticulum”J of Virology67(8):4972-4980 (1993).
Flores et al. “Akt-Mediated Survival of Oligodendrocytes Induced by Neuregulins”J of Neuroscience20(20):7622-7630 (2000).
Geballe et al. “Variable inhibition of cell-free translation by HIV-1 transcript leader sequences”Nucleic Acids Research20(16):4291-4297 (1992).
Gelderblom et al. “Assembly and morphology of HIV: potential effect of structure on viral function”AIDS5:617-638 (1991).
Gheysen et al. “Assembly and Release of HIV-1 Precursor PR55gagVirus-like Particles from Recombinant Baculovirus-Infected Insect Cells”Cell59:103-112 (1989).
Glass et al. “A Sequence Related to the Human Gonadoliberin Precursor Near the N-termini of HIV and SIVgagPolyproteins”J. Theor. Biol. 150:489-496 (1991).
Görlich et al. “A protein of the endoplasmic reticulum involved early in polypeptide translocation”Nature357:47-52 (1992).
Göttlinger et al. “Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1”Proc. Natl. Acad. Sci. USA86:5781-5785 (1989).
Hockley et al. “comparative morphology of Gag protein structures produced by mutants of thegaggene of human immunodeficiency virus type 1”J. of General Virology75:2985-2997 (1994).
Isada, Carlos M. “New developments in long-term treatment of HIV: The honeymoon is over”Cleve. Clin. J. Med68:804-807 (2001).
Jain et al. “Metabolic complications associated with antiretroviral therapy”Antiviral Research51:151-177 (2001).
Jowett et al. “Distinct signals in human immunodeficiency virus type 1Pr55 necessary for RNA binding and particle formation”J. of General Virology73:3079-3086 (1992).
Kaushik et al. “Role of Glutamine-151 of Human Immunodeficiency Virus Type-1 Reverse Transcriptase in RNA-Directed DNA Synthesis”Biochemistry36:14430-14438 (1997).
Laughrea et al. “Mutations in the Kissing-Loop Hairpin of Human Immunodeficiency Virus Type 1 Reduce Viral Infectivity as well as Genomic RNA Packaging and Dimerization”J. of Virology71(5):3397-3406 (1997).
Li et al. “Multiple Forms of tRNALys3in HIV-1”Biochem&Biophysical Res. Comm. 227:530-540 (1996).
Lin et al. “Current Treatment Strategies for Chronic Hepatitis B and C”Annu. Rev. Med.52:29-49 (2001).
Lingappa et al. “A Eukaryotic Cytosolic Chaperonin Is Associated with a High Molecular Weight Intermediate in the Assembly of Hepatitis B Virus Capsid, a Multimeric Particle”J. of Cell Biology125(1):99-111 (1994).
Lingappa et al. “A Multistep, ATP-dependent Pathway for Assembly of Human Immunodeficiency Virus Capsids in a Cell-free System”J. Cell Biol.136:567-581 (1997).
Lo et al. “Interaction between Hepatitis C Virus Core Protein and E1 Envelope Protein”J. of Virology70(8):5177-5182 (1996).
Ohlmann et al. An Internal Ribosome Entry Segment Promotes Translation of the Simian Immunodeficiency Virus Genomic RNAJ. of Bio. Chem276(16):11899-11906 (2000).
Page et al. “Construction and Use of a Human Immunodeficiency Virus Vector for Analysis of Virus Infectivity”J. of Virology64(11):5270-5276 (1990).
Papadopulos-Eleopulos, et al. “A critical analysis of the pharmacology of AZXT and its use in AIDS”Curr. Med. Res. Opin.15 Suppl. 1:S1-45 (1999) [Abstract Only].
Ren, et al. “Caco-2 cell permeability vs human gastro-intestinal absorption: QSPR analysis”Prog. Drug. Res. Spec.No. 1-34 (2001).
Richards et al. “Inhibition of the aspartic proteinase from HIV-2”FEBS Lett.253:214-216 (1989).
Sakallan et al. “Synthesis and Assembly of Retrovirus Gag Precursors into Immature Capsids In Vitro”J. of Virology70:3706-3715 (1996).
Santolini et al. “Biosynthesis and Biochemical Properties of the Hepatitis C Virus Core Protein”J. of Virology68(6):3631-3641 (1994).
Sen et al. “The Interferon System—A Bird's Eye View of Its Biochemistry”J. of Biological Chemistry267(8):5017-5020 (1992).
Shih et al. “Modulation of thetrans-Suppression Activity of Hepatitis C Virus Core Protein by Phosphorylation”J. of Virology69(2):1160-1171 (1995).
Smith, Kendall A. “To cure chronic HIV infection, a new therapeutic strategy is needed” Curr. Opin. Immunol. 13:614-624 (2001).
Spirin et al. “A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield”Science242:1162-1164 (1988).
Tanchou et al. “Formation of Stable and Functional HIV-1 Nucleoprotein Complexes in Vitro”J. Mol. Biol.252:563-571 (1995).
Towler et al. “The Biology and Enzymology of Eukaryotic Protein Acylation”Ann. Rev. Biochem.57:69-99 (1988).
Trono et al. “HIV-1 Gag Mutants Can Dominantly Interfere with the Replication of the Wild-Type Virus”Cell59:113-120 (1969).
VanLint, et al. “Transcription Factor

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