Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1993-12-29
1998-09-22
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
536 2372, 536 241, 435 691, 4351723, 4352351, 4353201, 435419, A01H 500, C12N 1540, C12N 1583, C12N 504
Patent
active
058116532
ABSTRACT:
A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.
Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.
REFERENCES:
Raffo, Anthony J. and Dawson, William O. "Constructon of Tobacco Mosaic Virus Subgenomic Replicons That Are Replicated and Spread Systemically in Tobacco Plants". Virology184: 277-289 (1991).
Joshi, Rajiv L. et al. "BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells". The EMBO Journal, vol. 9: 2663-2669 (1990).
Joshi et al. 1991. FEBS Lett.: 1-8.
Takamatsu et al. 1987. EMBO J. 6: 307-11.
Larkins et al. 1985. J. Cell. Biochem. Suppl. O ( 9 Part C): 264.
Barton et al. 1987. Plant Physiol. 85:1103-9
Biosource Technologies, Inc.
Fox David T.
Halluin Albert P.
LandOfFree
Viral amplification of recombinant messenger RNA in transgenic p does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Viral amplification of recombinant messenger RNA in transgenic p, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Viral amplification of recombinant messenger RNA in transgenic p will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1623396