Viral amplification of recombinant messenger RNA in transgenic p

Details Viral amplification of recombinant messenger RNA in transgenic p Viral amplification of recombinant messenger RNA in transgenic p

1994-11-09

1999-10-12

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Nonplant protein is expressed from the polynucleotide

800298, 8003173, 435 691, 4352351, 4353201, 435468, 536 2372, A01H 500, C12N 1540, C12N 1583

Patent

active

059657944

ABSTRACT:
A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.
Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.

REFERENCES:
Mori et al. 1991. J. Gen. Virol. 72(2):243-6.
Mori et al. 1992. J. Gen. Virol. 73(1):169-172.
Takamatsu et al. 1987. EMBO J. 6(2):307-311.
Hoffman et al. 1987. EMBO J. 6: 3213-3221.
Ahlquist, P., et al., Viral Vectors, Cold Spring Harbor Laboratory, New York, 183-189 (1988).
Ahlquist, P., and Pacha, R.F., Physiol. Plant. 79:163-167 (1990).
Butler, P.J.G., Mayo, M.A., Molecular architecture and assembly of tobacco mosaic virus particles, The molecular biology of the positive strand RNA viruses. (D. J. Rowlands, M.A. Mayo, and B.W.J. Mahy, eds.), Academic Press, London. pp. 237-257 (1987).
Cassidy, B., and Nelson, R., Phytopathology 80:1037 (1990).
Chapman, S., et al., Plant Journal 2:549 (1992).
Citovsky, V., Zambryski, P., BioEssays 13:373-379 (1991).
Culver, J.N., Lehto, K., Close, S.M., and Dawson, W.O., Virology (in press).
Dawson, W.O. and Hilf, M.E., Ann. Rev. Plant Physiol. Plant Mol. Biol. 43:527-555 (1992).
Dawson, et al., Phytopahtol. 78:783-789 (1988).
Dawson, W.O. et al., Virology 172:285-292 (1989).
Dawson, W.O., Adv. Virus Res. 38:307-342 (1990).
Dawson, W.O., Virology 186:359-367 (1992).
Deom, C.M., Lapidot, M., and Beachy, R.N., Cell 69:221-224 (1992).
Deom, C.M., Oliver, M.J., and Beachy, R.N., Science 237:389-394 (1987).
Dolja, V., et al., PNAS 89:10208 (1992).
Donson et al., Proc. Natl. Acad. Sci. 88:7204-7208 (1991).
Dunsmuir, P., Bond, D., Lee, K., Gidoni, D., and Towsend, J., Stability of introduced genes and stability of expression, Plant molecular biology manual. (S.B. Gelvin, R.A. Schilperoort, and D.P.S. Verma, eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. C1:1-17 (1988).
French, R. et al., Science 231:1294-1297 (1986).
Horsch, R.B., Fry, J., Hoffmann, N., Neidermeyer, J., Rogers, S.G., and Fraley, R.T., Leaf disc transformation, Plant molecular biology manual. (S.B. Gelvin, R.A. Schilperoort, and D.P.S. Verma, eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. A5:1-9 (1988).
Joshi, R.L., and Joshi, V., FEBS Lett. 281:1-8 (1991).
Joshi, R.L., et al., EMBO J. 9:2663-2669 (1990).
Jupin, I., et al., Virology 178:273-280 (1990).
Kearney, C. M., Donson, J., Jones, G. E., and Dawson, W. O., Virology 192:000-000 (1993) (in press).
Martelli, G.P., Plant Disease 76:436 (1992).
Ogawa, T., Watanabe, Y., Meshi, T., and Okada, Y., Virology 185:580-584 (1991).
Ow, D. W., et al., Science 234:856 (1986).
Potrykus, I., Annual Rev. Plant Physiol. Plant Mol. Biol. 42:205-225 (1991).
Raffo A.J., and Dawson, W. O., Virology 184:277-289 (1991).
Rowlands, D.J., Mayo, M.A., and Mahy, B.W.J., eds., Academic Press, London. pp. 237-257 (1987).
Saito, T., Yamanaka, K., Okada, Y., Virology 176:329-336 (1990).
Shaw, W.V., Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria, Methods in Enzymology, vol. 53, (S. Fleischer and L. Packer, eds.), pp. 737-755 (1975).
Takamatsu, N., et al., J. Virol. 65:1619-1622 (1991).
Takamatsu, N., et al., J. Virol. 64:3686-3693 (1990).
Takamatsu, N., et al., EMBO J. 6:307-311 (1987).
Takamatsu, N., et al., FEBS Letters 269:73-76 (1990).
Turpen and Dawson, Transgenic Plants, Fundamentals and Applications, Marcel Dekkar, New York, pp. 195-217 (1992).
Turpen, T. H., Ph.D. Dissertation, University of California, Riverside, pp. 72-87 (1992).
Turpen, T., Ph.D. Dissertation, University of California, Riverside, pp. 106-132 (1992).
Turpen, T.H., and Dawson, W.O., Amplification, movement and expression of genes in plants by viral-based vectors, Transgenic plants: fundamentals and applications (A. Hiatt, ed.), Marcel Dekker, Inc., New York, pp. 195-217. (1992).
Van Haute, E., Joos, H., Maes, M., Warren, G., Van Montagu, M., and Schell, J., EMBO J. 2:411-417 (1983).
Velton and Schell, NAR 13:6981 (1985).
Walden and Schell, Eur. J. Biochem. 192:563-576 (1990).
Yamaya, J., Yoshioka, M., Meshi, T., Okada, Y., and Ohno, T., Mol. Gen. Genet. 211:520-525 (1988).
Zaitlin, M., Hull, R., Ann. Rev. Plant Physiol. 38:291-315 (1987).
Zambryski, P., Joos, H., Genetello, C., Leemans, J., Van Montagu, M., and Schell, J., EMBO J. 2:2143-2150 (1983).
Raffo, Anthony J. and Dawson, William O. "Constructon of Tobacco Mosaic Virus Subgenomic Replicons That Are Replicated and Spread Systemically in Tobacco Plants". Virology 184: 277-289 (1991).
Joshi, Rajiv L. et al. "BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells". The EMBO Journal, vol.9: 2663-2669 (1990).

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