Viable microorganism detection by induced fluorescence

Details Viable microorganism detection by induced fluorescence Viable microorganism detection by induced fluorescence

1988-07-21

1992-02-18

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 14, 435 18, 435 19, 435 21, 435 29, 435 34, C12Q 106

Patent

active

050893955

ABSTRACT:
The present invention concerns a spectrometric technique to determine microorganism detection and identification by taking advantage of the inherent extracellular enzymes present in living organisms, as opposed to dead, non-enzyme producing organisms. These enzymes are harnessed in the in vivo reactions with a non-fluorescent dye containing a select organic functional group that is known to be cleaved or hydrolyzed by the certain enzyme. The dye is tailored such that one of the products fluoresces, so that by employing a conventional spectrofluorimeter, the rate of fluorescence can be determined. By subjecting a plurality of samples having different cellular concentrations of viable microorganisms to the same non-fluorescent dye, or by subjecting the same bacterial sample to a number of different non-fluorescent dyes, a pattern of fluorescent rates emerge. By employing the pattern recognition set to standard microorganism fluorescent response curves, microorganism detection and identification can be determined. Thus, the present invention concerns a process for determining microorganism detection, identification and concentration.

REFERENCES:
patent: 4283490 (1981-08-01), Plakas
Snyder et al., Biotechnology and Bioengineering, 26: 1395-1397, "Viable Microorganism Detection by Induced Fluorescence", Nov. 1984.
Godsey et al. (1981) Journal of Chemical Microbiology vol. 13, No. 3, pp. 483-490.
Bailey et al., Diagnostic Microbiology, pp. 72-73, 1966.
The Fisher Scientific Catalog, 1983 Edition pp. 286-287 and 326.

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