Viable cell labelling

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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424 3, 424 71, 424 11, 424 51, 424 9, 424 52, 435 35, 514 5, 514735, 430578, 544253, C12Q 104, C12Q 116

Patent

active

047834013

ABSTRACT:
Methods for reproducibly labelling viable cells with cyanine dyes that do not significantly affect cell viability. Applications for labelled cells include using labelled red blood cells to distinguish post-transfusional bleeding from immunologic reaction and using dilution to measure growth rate of cultured cells.

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Sims, P. J., Waggoner, A. S., Wang, C-H, and Hoffman, J. F.: Studies on the Mechanism by which Cyanine Dyes Measure Membrane Potential in Red Blood Cells and Phosphadidyl Doline Vesicle. Biochemistry, vol. 13(16) 3315-3330 (1974).
Jakoby, W. B. & Pastan, I. H.: Cell Culture (Methods in Enzymology) Academic Press (N.Y., San Francisco, London) 1979, pp. 52, 73, 136-137.
Stedman's Medical Dictionary, Williams & Willcins, 1980 p. 732.

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