Veterinary pharmaceutical formulation containing avian embryonic

Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Embryonic structure

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435349, 424 937, A61K 3554, A61K 3512

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active

058305106

ABSTRACT:
A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype is disclosed. The method comprises collecting avian cells from an avian blastoderm prior to formation of the primitive streak, then depositing the avian cells in contact with a mouse fibroblast feeder cell layer, and then growing the avian cells on the mouse fibroblast feeder cell layer in the presence of a media containing leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture. Cell cultures produced by the aforesaid process and veterinary pharmaceutical formulations containing such cells are also disclosed.

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