Vesticles and use thereof in an enzyme assay

Details Vesticles and use thereof in an enzyme assay Vesticles and use thereof in an enzyme assay

1985-05-28

1989-05-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 19, 435 21, 435810, 436501, 436518, 436536, 436808, 436829, G01N 33543

Patent

active

048289825

ABSTRACT:
A sac is produced by use of a compound which includes a hydrophobic portion and a polar group which is hydrolyzable by an enzyme and which is spaced from the hydrophobic portion by a hydrophilic portion which does not have a polarity sufficient to form a vesicle. A detectable marker may be released from the sac by use of a hydrolase enzyme, whereby the sac may be used as a substrate in an enzyme assay.

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Mahler et al., Biological Chemistry, Harper & Row Publishers, Inc., New York, 1966, pp. 511-513.
McDougall et al., Proc. Nat. Acad. Sci. (U.S.A.), 71, 3487-3491, 1974.
Engvall et al., in Feldmann et al., Ed., First International Symposium on Immunoenzymatic Techniques, North-Holland Publishing Company, Amsterdam, 1976, pp. 135-147.
R. Neumann et al., Chem. Abs. 104, 110158f, 1986.

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