Vesicle membrane transport proteins

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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530350, 530827, C07H 2104, C07K 14435

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active

056889364

ABSTRACT:
Complete cDNA and amino acid sequences are disclosed for rat adrenal-specific and brain-specific transport protein, as well as for human brain-specific transport protein. Methods for obtaining the genes encoding these proteins and for obtaining recombinantly produced protein are described. Antibodies and methods for isolating additional vesicle membrane transport proteins are also described. Methods for using the vesicle membrane transport proteins to identify compounds that selectively inhibit transport of toxic molecules into vesicles, and that prevent inhibition of transport of toxic molecules are also provided. The invention includes methods to treat and diagnose diseases associated with sequestration of toxic molecules in mammalian cells.

REFERENCES:
patent: 4376110 (1983-03-01), David et al.
patent: 4548904 (1985-10-01), Kent et al.
patent: 5082670 (1992-01-01), Gage et al.
P.M. Burger et al., "GABA and Glycine in Synaptic Vesicles: Storage and Transport Characteristics," Neuron 7:287-293 (1991).
J.W. Hell et al., "Energy Dependence and Functional Reconstitution of the .gamma.-Aminobutyric Acid Carrier from Synaptic Vesicles," J. Biol. Chem. 265:2111-2117 (1990).
P.R. Maycox et al., "Glutamate Uptake by Brain Synaptic Vesicles," J. Biol. Chem. 263:15423-15428 (1988).
B.W. Hicks et al., "Purification and Characterization of a Nonvesicular Vesamicol-Binding Protein from Electric Organ and Demonstration of a Related Protein in Mammalian Brain," J. Neurochem. 57:509-519 (1991).
P.E. Kish et al., "Active Transport of .gamma.-Aminobutyric Acid and Glycine into Synaptic Vesicles," Proc. Natl. Acad. Sci. USA 86:3877-3881 (1989).
M.D. Carlson et al., "Characterization of the Solubilized and Reconstituted ATP-Dependent Vesicular Glutamate Uptake System," J. Biol. Chem. 264:7369-7376 (1989).
Y. Stern-Bach et al., "Identification and Purification of a Functional Amine Transporter from Bovine Chromaffin Granules," J. Biol. Chem. 265:3961-3966 (1990).
D.B. Calne & J.W. Langston, "Aetiology of Parkinson's Disease," Lancet 1457-1459 (Dec. 24/31, 1983).
J.W. Langston et al., "Chronic Parkinsonism in Humans Due to a Product of Meperidine-Analog Synthesis," Science 219:979-980 (1983).
R.S. Burns et al., "A Primate Model of Parkinsonism: Selective Destruction of Dopaminergic Neurons in the Pars Compacta of the Substantia Nigra by N-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine," Proc. Natl. Acad. Sci. USA 80:4546-4550 (1983).
R.E. Heikkila et al., "Dopaminergic Neurotoxicity of 1-Methyl-4-Phenyl-1,2,5,6-Tetrahydropyridine in Mice," Science 224:1451-1453 (1984).
K. Chiba et al., "Metabolism of the Neurotoxic Tertiary Amine, MPTP, by Brain Monoamine Oxidase," Biochem. Biophys. Res. Comm. 120:574-578 (1984).
J.W. Langston et al., "Pargyline Prevents MPTP-Induced Parkinsonism in Primates," Science 225:1480-1482 (1984).
S.P. Markey et al., "Intraneuronal Generation of a Pyridinium Metabolite May Cause Drug-Induced Parkinsonism," Nature 311:464-467 (1984).
J.A. Javitch et al., "Parkinsonism-Inducing Neurotoxin, N-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine: Uptake of the Metabolite N-Methyl-4-Phenylpyridine by Dopamine Neurons Explains Selective Toxicity," Proc. Natl. Acad. Sci. USA 82:2173-2177 (1985).
S.H. Snyder et al., "Selective Uptake of MPP.sup.+ by Dopamine Neurons is Required for MPTP Toxicity: Studies in Brain Synaptosomes and PC-12 Cells," MPTP: A Neurotoxin Producing a Parkinsonian Syndrome (S.P. Markey et al., eds., Academic Press, New York, 1986), pp. 191-201.
M.J. Krueger et al., "Evidence of the Blockade of Mitochondrial Respiration by the Neurotoxin 1-Methyl-4-Phenylpyridinium (MPP.sup.+) Involves Binding at the Same Site as the Respiratory Inhibitor, Rotenone," Biochem. Biophys. Res. Comm. 169:123-128 (1990).
R.R. Ramsay et al., "Interaction of 1-Methyl-4-Phenylpyridinium Ion (MPP.sup.+) and Its Analogs with the Rotenone/Piericidin Binding Site of NADH Dehydrogenase," J. Neurochem. 56:1184-1190 (1991).
Y. Mizuno et al., "Deficiencies in Complex I Subunits of the Respiratory Chain in Parkinson's Disease," Biochem. Biophys. Res. Comm. 163:1450-1455 (1989).
W.D. Parker, Jr. et al., "Abnormalities of the Electron Transport Chain in Idiopathic Parkinson's Disease," Ann. Neurol. 26:719-723 (1989).
J.M. Shoffner et al., "Mitochondrial Oxidative Phosphorylation Defects in Parkinson's Disease," Ann. Neurol. 30:332-339 (1991).
The Parkinson's Study Group, "Effect of Deprenyl on the Progression of Disability in Early Parkinson's Disease," New Engl. J. Med. 321:1364-1371 (1989).
G. Cohen, "Monoamine Oxidase and Oxidative Stress at Dopaminergic Synapses," J. Neural Transm. Suppl. 32:229-238 (1990).
L. Turski et al., "Protection of Substantia Nigra from MPP.sup.+ Neurotoxicity by N-Methyl-D-Aspartate Antagonists," Nature 349:414-418 (1991).
C. Hyman et al., "BDNF is a Neurotrophic Factor for Dopaminergic Neurons of the Substantia Nigra," Nature 350:230-232 (1991).
J.F. Reinhard, Jr. et al., "Subcellular Compartmentalization of 1-Methyl-4-Phenylpyridinium with Catecholamines in Adrenal Medullary Chromaffin Vesicles may explain the lack of Toxicity to Adrenal Chromaffin Cells," Natl. Acad. Sci. USA 84:8160-8164 (1987).
L.A. Greene & G. Rein, "Release, Storage, and Uptake of Catecholamines by a Clonal Cell Line of Nerve Growth Factor (NGF) Responsive Pheochromocytoma Cells," Brain Res. 129:247-263 (1977).
T. Denton & B.D. Howard, "A Dopaminergic Cell Line Variant Resistant to the Neurotoxin 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine," J. Neurochem. 49:622-630 (1987).
D.W. Choi & S.M. Rothman, "The Role of Glutamate Neurotoxicity in Hypoxic-Ischemic Neuronal Death," Annu. Rev. Neurosci. 13:171-182 (1990).
A. Carlsson, "Early Psychopharmacology and the Rise of Modern Brain Research," J. Psychopharmacol. 4:120-126 (1990).
R.J. Wyatt, "Schizophrenia, Just the Facts," Schizophr. Res. 1:3-18 (1988).
R.G. Johnson, Jr., "Accumulation of Biological Amines Into Chromaffin Granules: A Model for Hormone and Neurotransmitter Transport," Physiol. Rev. 68:232-307 (1988). H! Acetylcholine by Torpedo Electric Organ Synaptic Vesicles," Biochemistry 21:3037-3043 (1982).
J.W. Hell et al., "Uptake of GABA by Rat Brain Synaptic Vesicles Isolated by a New Procedure," EMBO J. 7:3023-3029 (1988).
P.R. Maycox et al., "Bacteriorhodopsin Drives the Glutamate Transporter of Synaptic Vesicles After Co-reconstitution," EMBO J. 9:1465-1469 (1990).
J.W. Hell et al., "Functional Reconstitution of .gamma.-Aminobutyric Acid Transporter from Synaptic Vesicles Using Artificial Ion Gradients," Biochemistry 30:11795-11800 (1991).
C. Chen & H. Okayama, "High-Efficiency Transformation of Mammalian Cells by Plasmid DNA," Mol. Cell. Biol. 7:2745-2752 (1987).
A. Aruffo & B. Seed, "Molecular Cloning of CD28 cDNA by a High-Efficiency COS Cell Expression System," Proc. Natl. Acad. Sci. USA 84:8573-8577 (1987).
A.T. Dobson et al., "A Latent Nonpathogenic HSV-1 Derived Vector Stably Expresses .beta.-Galactosidase in Mouse Neurons," Neuron 5:353-360 (1990).
A.I. Geller & A. Freese, "Infection of Cultured Central Nervous System Neurons with a Defective Herpes Simplex Virus 1 Vector Results in Stable Expression of Escherichia coli .beta.-Galactosidase," Proc. Natl. Acad. Sci. USA 87:1149-1153 (1990).
J.F. Reinhard, Jr. et al., "Mechanisms of Toxicity and Cellular Resistance to 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine and 1-Methyl-4-Phenylpyridinium in Adrenomullary Chromaffin Cell Cultures," J. Neurochem. 55:311-320 (1990).
M. Kozak, "Compilation and Analysis of Sequences Upstream from the Translational Start Site in Eukaryotic mRNAs," Nucl. Acids Res. 12:857-872 (1984).
D. Eisenberg et al., "Analysis of Membrane and Surface Protein Sequences with the Hydrophobic Moment Plot," J. Mol. Biol. 179:125-142 (1984).
J. Guastella et al., "Cloning and Expression of a Rat Brain GABA Transporter," Science 249:1303-1306 (1990).
T. Pacholczyk et al., "Expression Cloning of a Cocaine-and Antidepressant-Sensitive Human Noradrenaline Transporter," Nature 350:350-354 (1991).
S. Shimada et al., "Cloning and Expression of a Cocaine-Sensitive Dopamine Transporte

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