Very rapid detection of fungal infections

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 34, 435 19, 435 30, 435 29, C12Q 128, C12Q 144, C12Q 102, C12Q 104

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050988301

ABSTRACT:
A method for the detection of a fungal infection within 1-2 hours comprises the steps of: (a) contacting a sample suspected of containing a peroxidase-containing fungus with a mixture which comprises (i) a peroxide from hydrogen peroxide and addition compounds thereof containing latent hydrogen peroxide; (ii) a scavenger to remove heavy metals which may potentially decompose said peroxide catalytically; (iii) an alkaline buffer solution having the capacity to maintain the pH at a value within the range of 9.5 to 14; and (iv) a colorless oxidizable substrate (such as tyrosine, .beta.-(3,4-dihydroxyphenyl)-.alpha.-alamine (DOPA) or caffeic acid), which on reaction with oxygen at said pH value gives a visibly colored oxidation product; and (b) observing whether a color develops indicating the presence of peroxidase-containing fungus in the sample. Also included is a test kit for carrying out the method of the invention.

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Huxham et al. (1986) A simple usual method for assessing the actuation . . . insect haemocytes in vitro, J Immo. Meth 94: 271-7.

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