Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1990-07-16
1992-03-24
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 34, 435 19, 435 30, 435 29, C12Q 128, C12Q 144, C12Q 102, C12Q 104
Patent
active
050988301
ABSTRACT:
A method for the detection of a fungal infection within 1-2 hours comprises the steps of: (a) contacting a sample suspected of containing a peroxidase-containing fungus with a mixture which comprises (i) a peroxide from hydrogen peroxide and addition compounds thereof containing latent hydrogen peroxide; (ii) a scavenger to remove heavy metals which may potentially decompose said peroxide catalytically; (iii) an alkaline buffer solution having the capacity to maintain the pH at a value within the range of 9.5 to 14; and (iv) a colorless oxidizable substrate (such as tyrosine, .beta.-(3,4-dihydroxyphenyl)-.alpha.-alamine (DOPA) or caffeic acid), which on reaction with oxygen at said pH value gives a visibly colored oxidation product; and (b) observing whether a color develops indicating the presence of peroxidase-containing fungus in the sample. Also included is a test kit for carrying out the method of the invention.
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Huxham et al. (1986) A simple usual method for assessing the actuation . . . insect haemocytes in vitro, J Immo. Meth 94: 271-7.
Bar-Or David
Solomons Clive
Diagnostic Markers, Inc.
Preston David R.
Wax Robert A.
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