Very low protein nutrient medium for cell culture

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352403, 528 49, C12N 500, C08G 1808

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active

051358662

ABSTRACT:
This nutrient medium is very effective for the serum-free or serum-protein-free culture of various animal cells, in both high and low density culture. Serum proteins have been replaced with non-protein-based cell growth enhancers and a non-serum derived protein supplement. The non-protein growth enhancer is a modified or derivatized polyurethane prepolymer of polymer and preferably is a sulfhydryl derivative of polyurethane. The protein supplement may be insulin, an insulin analog or an insulin-like growth factor.

REFERENCES:
patent: 3450598 (1969-06-01), Welsh et al.
patent: 3723393 (1973-03-01), Kistner
patent: 3939123 (1980-02-01), Matthews
patent: 4038139 (1977-07-01), Birch
patent: 4072565 (1978-02-01), Weiss et al.
patent: 4137200 (1979-01-01), Wood et al.
patent: 4177038 (1979-12-01), Biebricher et al.
patent: 4182827 (1980-01-01), Jones
patent: 4226935 (1980-10-01), Fusee
patent: 4241537 (1980-12-01), Wood
patent: 4293679 (1981-10-01), Cogliano et al.
patent: 4423145 (1983-12-01), Stampfer
patent: 4439585 (1984-03-01), Gould et al.
patent: 4443546 (1984-04-01), Stemerman et al.
patent: 4452893 (1984-06-01), Ng et al.
patent: 4485227 (1984-11-01), Fox
patent: 4560655 (1985-12-01), Baker
patent: 4569981 (1986-03-01), Wenzel et al.
patent: 4657866 (1987-04-01), Kumar
patent: 4681851 (1987-07-01), Baumgarten et al.
patent: 4767704 (1988-08-01), Cleveland et al.
patent: 4786599 (1988-11-01), Chessebeuf et al.
patent: 4851346 (1989-07-01), Chan
patent: 4929706 (1990-05-01), Heifetz et al.
patent: 4940737 (1990-07-01), Braatz et al.
Velez et al., "Kinetics of Monoclonal Antibody Production in Low Serum Growth Medium", J. of Immun. Methods, vol. 86, pp. 45-52 (1986).
Schacter, "Serum Free Medium for Growth Factor Dependent and Independent Plasma Cytomas and Hybridomas", J. of Immon. Methods, vol. 99, pp. 259-270 (1987).
Mizrachi, "Primatone RL in Mammalian Cell Culture Media", Biotechnol. and Bioengineering, vol. 19, pp. 1557-1561 (1977).
Mizrahi, "Pluronic Polyols in Human Lymphocyte Cell Line Cultures", J. of Clinical Microbiol. vol. 2, pp. 11-13 (1975).
"Pluronic Polyols in Cosmetics", a brochure published by Wyandotte Chem. Corp. 1979.
Murhammer et al., "Scale-Up of Insect Cell Cultures: Protective Effects of Pluronic F-68" Biotechnology vol. 6, pp. 1411-1418.
Wolfe et al., Biotechniques, vol. 6, pp. 62-67 (1988).
Smith et al., J. Amer. Chem. Soc., vol. 81 (1959) pp. 161-163.
Farkas et al., Advan. Catalysis, vol. 13 (1962) pp. 434-439.
Ulrich et al., J. Org. Chem., vol. 32, pp. 3938-3941 (1967).
Freshney, "Culture of Animal Cells; A Manual of Basic Technique", Chapter 9, pp. 67-78 (1983).
Iscove et al., "Complete Replacement of Serum by Albumin, Transferrin, and Soybean Lipid in Cultures of Lipopolysaccharide-Reactive B Lymphocytes"; J. Exp. Med.; vol. 147/pp. 923-933 (1978).
Ham et al., "Media and Growth Requirements (I.A.5)"; Methods in Enzymology; (1978).
Barnes et al., "Methods for Growth of Cultured Cells in Serum-Free Medium"; Analytical Biochemistry; vol. 102/pp. 255-270 (1980).
McHugh et al., "Serum-Free Growth of Murine and Human Lymphoid and Hybridoma Cell Lines"; BioTechniques; pp. 72-77 (Jun. 1983).
KC Biological, "KC2000.TM. Serum-Independent Medium"; Technical Informatoin Bulletin #24.
KC Biological, "KC2000.TM. Serum-Independent Medium"; Technical Information Bulletin #25.
Ventrex, "Completely Defined Serum Free Media" (Brochure) (1984).
Wolfe et al., "A New Serum-Free Medium for Monoclonal Antibody Production"; BioTechniques; vol. 6, No. 1/pp. 62-67; (1988).
Kane et al., "Protein-Free Culture Medium Containing Polyvinyl-Alcohol, Vitamins, and Amino-Acids Supports Development of Eight-Cell Hamster Embryos to Hatching Blastocysts," Journal of Experimental Zoology, vol. 247, pp. 183-187 (1988).
Wolfe et al., "Continuous Culture of Rat C6 Glioma in Serum-Free Medium", Journal of Cell Biology; vol. 87/pp. 434-441 (1980).
Barnes et al., "Serum-Free Cell Culture: a Unifying Approach"; Cell; vol. 22/pp. 649-655 (Dec., 1980).
Wolfe et al., "Continuous Serum-Free Culture of the N18TG-2 Neuroblastoma x Glioma Hybrid Cell Lines"; Cold Spring Harbor Conferences on Cell Proliferation; vol. 9/pp. 1075-1088 (1982).

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