Vertical gel slab electrophoresis method

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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Details

G01N 2726, G01N 2728

Patent

active

042908710

ABSTRACT:
A vertical gel electrophoresis apparatus includes a U-shaped structure consisting of a container for upper buffer solution and extending parallel plate sample units which form the legs of the U. In the casting mode the U-shaped structure is inverted with the tank resting on the ground surface and with the bottom ends of the sample units being sealed against the sealing strip on the top of the container. After the sample units are filled to the top and the gel polymerized (with the use of a comblike well forming structure if needed) the sample units are flipped and reinserted. However because of the offset of the plates of the sample units in the lateral direction the formerly sealed end of the sample plates is now exposed to a slot or aperture which extends into the upper buffer solution container. The entire U-shaped structure rests in a tank of lower buffer solution on a slab type heat exchanger where cooling can occur on both sides of the sample units.

REFERENCES:
patent: 4035377 (1977-07-01), Detroy
patent: 4169036 (1979-09-01), Anderson et al.

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