Vertical gel slab electrophoresis apparatus and method therefor

Details Vertical gel slab electrophoresis apparatus and method therefor Vertical gel slab electrophoresis apparatus and method therefor

1981-01-30

1982-04-20

Williams, Howard S.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, B01D 5702, G01N 2726

Patent

active

043257968

ABSTRACT:
A vertical gel slab electrophoresis apparatus includes a pair of sample units (12, 13) each of which is a pair of clamped together, spaced glass plates (23a, 23b and 24a, 24b) for containing the gel (21, 22). A casting stand is provided which holds the pair of sample units vertical and seals their bottom slots (26, 27) to prevent leakage of the gel. After polymerization, an upper buffer solution container (33) is locked on to the pair of sample units, the casting stand removed, and the remaining U-shaped structure placed in a tank (11) where electrophoresis may be initiated.
A specialized seal is provided between the sample units and the upper buffer chamber (33) in the form of a resilient gasket (36, 37) stretched over the one end of a pair of sample plates and this is then wedged into a mated truncated conical sleeve extending from the bottom (34) of the upper buffer container to provide a seal which effectively isolates the upper buffer solution (32) from the lower buffer solution (28).

REFERENCES:
patent: 3980540 (1976-09-01), Hoefer
patent: 4035377 (1977-07-01), Detroy
patent: 4169036 (1979-09-01), Anderson et al.

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