Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1975-08-18
1976-09-14
Mack, John H.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726
Patent
active
039805409
ABSTRACT:
A gel electrophoresis device in which the separating gel is cast in a vertical position between hydrophilic plates which form a gel slab chamber filled with a separating gel solution from the bottom for reducing fill turbulence allowing formation of a gel slab having a predetermined gradient. A removable seal is applied to the bottom of the gel slab chamber for containing the separating gel solution prior to polymerization. One gel slab chamber may be removably attached to each vertical side of a central temperature control chamber which is completely enclosed and which has an inlet and an outlet channel for circulation of temperature control fluid therethrough. An upper buffer chamber which opens upwardly is formed above the central temperature control chamber in communication with the top of the vertical gel slab chambers. The top of the gel slab is formed using a well forming implement made of polymerization inhibiting material so that the implement may be withdrawn after polymerization without altering the desired shape of the top of the vertical gel slab. The gel slab chambers attached to the central enclosed temperature control and upper buffer chamber are lowered into an outer chamber after polymerization of the separating gel solution and removal of the seals from the lower end of the gel slab chambers. The outer container is filled with a buffer solution in consequent directi contact with the lower end of the gel slabs, and the upper buffer chamber is filled with a buffer solution in direct contact with the top of the gel slabs. Samples are underlaid beneath the upper buffer solution atop the shaped upper surface of the gel slabs and a cover is placed on the outer container to contact a pair of electrodes which are in separate electrical contact with one of the upper and lower buffer chambers for applying a voltage therebetween, thereby causing electrophoretic separation of the sample within the gel slab.
REFERENCES:
patent: 3129158 (1964-04-01), Raymond et al.
patent: 3799863 (1974-03-01), Zeineh
patent: 3879280 (1975-04-01), Peterson et al.
Hoefer Scientific Instruments
Mack John H.
Prescott A. C.
LandOfFree
Vertical gel slab electrophoresis apparatus and method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Vertical gel slab electrophoresis apparatus and method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vertical gel slab electrophoresis apparatus and method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1025345