Vertical electrophoresis system

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S456000, C204S465000, C204S466000, C204S469000, C204S470000, C204S606000, C204S615000, C204S616000, C204S618000, C204S619000, C204S620000

Reexamination Certificate

active

06942775

ABSTRACT:
A plugging medium is used to block the bottom opening of cassettes for vertical electrophoresis in order to facilitate filling the cassettes with separation media. Cassettes can be filled within a vertical electrophoresis system in which electrophoresis is conducted without further manipulation of the cassettes. The system includes a frame assembly mounted on a base containing a basin for plugging solution. The cassette is mounted to the frame assembly in a substantially vertical position such that a bottom opening of the empty cassette resides below the rim of the basin. The plugging solution (e.g. agarose gel) forms a plug within the bottom of the cassette to contain the separation medium.

REFERENCES:
patent: 3980540 (1976-09-01), Hoefer
patent: 4560459 (1985-12-01), Hoefer
patent: 4574040 (1986-03-01), Delony et al.
patent: 4954236 (1990-09-01), Kushner et al.
patent: 4957613 (1990-09-01), Schuette
patent: 5709788 (1998-01-01), Chen
patent: 5882495 (1999-03-01), Garrels
patent: 6013165 (2000-01-01), Wiktorowicz et al.
patent: 6162342 (2000-12-01), Perez et al.
patent: 2004/0045829 (2004-03-01), Ingenhoven et al.
patent: 299 22 680 (2000-03-01), None
patent: WO 94/29005 (1994-12-01), None
patent: 98/52031 (1998-11-01), None
patent: WO 98/52031 (1998-11-01), None
patent: WO 00/47984 (2000-08-01), None
patent: 02/21116 (2002-03-01), None
“CLP Mini2 Vertical Gel System” Conlab web site, admitted prior art.
“SERVA BlueVertical Slab Gel System 160/C”, Serva web site, admitted prior art.
“Mini-PROTEAN 3 Electrophoresis System”, Bio-Rad Laboratories web site, admitted prior art.

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