Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1989-11-27
1993-01-19
Moskowitz:, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
5303879, 5303882, 5303891, 536 235, C07K 300, C07H 1512
Patent
active
051808080
ABSTRACT:
The invention relates to the fibroblast proteoglycan, versican. The versican core protein has the amino acid sequence, and is encoded by the nucleotide sequence, as shown in FIG. 1. The nucleotide sequence and method of hyaluronic acid binding domain is provided and methods of preparing recombinant proteins having hyaluronic acid binding activity are provided. Such protein can be used to determine the presence of hyaluronic acid and as a vehicle to bring other molecules in contact with hyaluronic acid. The complete versican sequence will allow the production of the entire versican molecule to be used, for example, in tissue reconstruction. Nucleic acid probes are provided which are useful for detecting nucleic acid sequences encoding versican. The invention also provides antibodies reactive with versican.
REFERENCES:
Zimmermann et al. EMBO 8(10), 1989, pp. 2975-2982; abstract only.
Perides et al., Isolation and Partial Characterization of a Glial Hyaluronate-binding Protein, The Journal of Biological Chemistry, 264:5981-5987 (1989).
Saunders et al., Molecular Cloning of Syndecan, an Integral Membrane Proteoglycan, The Journal of Cell Biology 108:1547-1556 (1989).
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Kimata et al, A Large Chondroitin Sulfate Proteoglycan (PG-M) Synthesized before Chondrogenesis in the Limb Bud of Chick Embryo, The Journal of Biological Chemistry 261:13517-13525 (1986).
Yamagata et al., Chondroitin Sulfate Proteoglycan (PG-M-like Proteoglycan) Is Involved in the Binding of Hyaluronic Acid to Cellular Fibronectin, The Journal of Biological Chemistry 261:13526-13535 (1986).
Sai et al, Cloning and sequence analysis of a partial cDNA for chicken cartilage proteoglycan core protein, Proc. Natl. Acad. Sci. USA 83:5081-5085 (1986).
Deak et al., Complete amino acid sequence of chicken cartilage link protein deduced from cDNA clones, Proc. Natl. Acad. Sci. USA 83:3766-3770 (1986).
Johansson et al., Structure and interactions of proteoglycans in the extracellular matrix produced by cultured human fibroblasts, Biochem. J. 232:161-168 (1985).
La Jolla Cancer Research Foundation
Moskowitz: Margaret
Sisson Bradley L.
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