Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
1993-04-08
2004-03-23
Wortman, Donna C. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S070210, C435S339100, C435S346000, C424S147100, C530S388350, C530S413000
Reexamination Certificate
active
06709811
ABSTRACT:
The present invention is related generally to the preparation of diagnostic reagents. More particularly, the present invention is related to the preparation of an immunological reagent including a monoclonal antibody (MAb) reactive with an epitope found universally in all murine leukemia viruses (MuLVs) with only a few exceptions.
A variety of immunological reagents including MAbs directed at the proteins of MuLVs are known. These include MAbs specific for certain ecotropic, xenotropic and polytropic MuLVs from inbred mice and MAbs reactive with ecotropic viruses of feral mice. The antibodies have been employed in serological typing of different MuLVs, in histological localization of MuLV gene products in infected tissues; in flow cytometry to quantitate MuLV gene expression on the surface of cells; in radioimmune precipitation and immunoblotting of virally encoded proteins; in virus neutralization, and in quantitative assays of different types of MuLVs in complex mixtures of viruses.
However, MAbs differ greatly with respect to their applicability to the various procedures noted above. Those which efficiently detect cell surface antigen may not efficiently precipitate proteins or react in immunoblots. Some of the MAbs which react strongly with live cells, react poorly with fixed histological sections, and few of the MAbs exhibit a marked viral neutralizing activity even though many of them are reactive with viral envelope glycoproteins. Most of the available MAbs are reactive with only limited groups of viruses.
A versatile method for identifying the presence of virtually all classes, groups or strains of MuLVs has not heretofore been known or described.
SUMMARY OF THE INVENTION
It is, therefore, an object of the present invention to provide a versatile method for detecting MuLVs belonging to any or all of the ecotropic, xenotropic, polytropic as well as the amphotropic class of MuLVs.
It is a further object of the present invention to provide an immunological reagent of broad applicability for MuLVs including focal immunofluorescence assays on live or fixed monolayers, immunoblotting, immunoprecipitation, immunohistochemical, flow cytometric procedures and the like.
It is another object of the present invention to provide a method for effectively neutralizing MuLVs of virtually all classes.
It is an additional object of the present invention to provide a reliable method for screening cultures for the presence of MuLV.
Various other objects and advantages will become evident from the following detailed description of the invention.
REFERENCES:
Harlow et al., Antibodies A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring, 1988, pp. 148-152 and 567-569.*
Britt et al., J. Immunol. 130: 2363-2367, 1983.*
Sevier et al., Clin. Chem. 27: 1797-1806. 1981.*
Gangemi, J.D., 9-(2-Phosphonylmethoxyethyl)Adenine in the Treatment of Murine Acquired Immunodeficiency Disease and Opportunistic Herpes Simplex Virus Infections, Anti-Microbial Agents and Chemotherapy (1989) 33:1865-1868.
Research Agreement for Material provided by NIAID, NIH to Kay Townsend, Mar. 17, 1988 for Tissue culture supernatant of monoclonal antibody 83A25.
Research Agreement for Material provided by NIAID, NIH to Paul Hoffman, Baltimore, Maryland, Apr. 14, 1988 for 83A25.
Research Agreement for Material provided by NIAID, NIH to David Onions, Apr. 8, 1988 for Monoclonal antibodies against murine retrovirus envelope proteins. University of Glasgow.
Research Agreement for Material provided by NIAID, NIH to Marguerite Hays, Veterans Admin., May 25, 1988 for monoclonal antibodies to murine leukemia virus envelope proteins.
Research Agreement for Material provided by NIAID, NIH to David Gangemi, Columbia, S.C., Feb. 10, 1989 for HeLa CD4 positive clone 6C cells-hybridomas 83A25, 514, 516, 7 also 18-7.
Research Agreement for Material provided by NIAID, NIH to Robert Cozens, Basle, Switzerland, Feb. 20, 1989 for hybridomas 83A25, 514, 48, 516, 7, also anti-p30 18-7.
Material Transfer Agreement to Carol Funk, Tucson, Arizona, Sep. 11, 1989 for 83A25.
Material Transfer Agreement to Alan Rein, Frederick Maryland, Nov. 6, 1989 for MAbs as tissue culture supernatant—MAbs 83A25, 514, Hy7, 516 and 502.
Material Transfer Agreement to Dr. Randy Hock, Charlottesville, Virginia, Nov. 8, 1989 for MAb 83A25.
Material Transfer Agreement to Dr. Sidney Grossberg, Milwaukee, Wisconsin, Jan. 17, 1990, for Monoclonal antibodies reactive toward MuLV envelope proteins.
Material Transfer Agreement to Igor Roninson, Chicago, Illinois, Nov. 13, 1989, for Mouse monoclonal antibody 83A25.
Material Transfer Agreement to Piechaczyk Marc, France, Nov. 16, 1989, for Monoclonal antibody 83A25 as tissue culter supernatant.
Material Transfer Agreement to Didier Trono, Cambridge, Massachusetts, Dec. 15, 1989 for Monoclonal antibody 83A25 as tissue culture supernatant.
Material Transfer Agreement to Victor Garcia, Seattle, Washington, Jan. 25, 1990, for MAb 83A25 in the form of tissue culture supernatant.
Britt William J
Evans Leonard H
The United States of America as represented by the Department of
Townsend and Townsend / and Crew LLP
Wortman Donna C.
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