Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2005-06-28
2005-06-28
Steadman, David J. (Department: 1652)
Chemistry: molecular biology and microbiology
Vector, per se
C536S023200, C536S023400, C536S023700
Reexamination Certificate
active
06911341
ABSTRACT:
Strains of genetically modified prokaryotic micro-organisms capable of expressing polypeptides having the enzyme activity of the enzymes uridine posphorylase (UdP) and purine nucleoside phosphorylase (PNP) are described; the strains in question can be used, both in the form of whole cells and in the form of crude or purified extracts, to catalyse transglycosylation reactions between a donor nucleoside and an acceptor base with particularly high yields. The associated plasmid vectors are also described.
REFERENCES:
patent: 4347315 (1982-08-01), Krenitsky et al.
patent: 4835104 (1989-05-01), Yokozeki et al.
patent: 5563049 (1996-10-01), Kojima et al.
patent: 6197552 (2001-03-01), Yokozeki et al.
patent: 0002192 (1979-06-01), None
patent: 0038568 (1981-10-01), None
patent: 06-253854 (1994-09-01), None
patent: 6-253854 (1994-09-01), None
Bulow et al. (1991) Trends Biotech 9:226-231.
Novagen 1997 Catalog, pp. 42-43 and 156.
Sambrook et al. “Molecular Cloning, A Laboratory Manual, Second Edition”, Cold Spring Harbor Press, 1989, pp. 1.5-1.6.
Ausubel et al. Current Protocols in Molecular Biology, John Wiley and Sons, Inc., 1995, p. 16.1.1.
Database GenBank Accession No. L08752, Apr. 1993.
Branden et al. “Introduction to Protein Structure”, Garland Publishing Inc., New York, 1991, p. 247.
Witkowski et al. (1999) Biochemistry 38:11643-11650.
Hershfield, M.S. et al.; “Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol”; Proceedings of the Ntional Academy of Science of USA, vol. 88, 1991, pps. 7185-7189.
Brykun, I.A. et al.; “Cloning theEscherichia coliuridine phosphorylase gene udp and its expression in the recombinant plasmids”; Genetika, vol. XXV, No. 10, 1989, pps. 1717-1724, (russian language).
Walton, L. et al.; “Nucleotide sequence of theEscherichia coliuridine phosphorylase (udp) gene”; Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6741.
Takehara, M. et al.; “Molecular cloning and nucleotide sequence of purine nucleoside phosphorylase and uridine phosphorylase genes fromKlebsiellasp.”, Bioscience, Biotechnology and Biochemistry, vol. 59, No. 10, 1995, pps. 1987-1990.
Krenitsky, T.A. et al.; “Purine nucleoside synthesis, an efficient method employing nucleoside phosphorylases”, Biochemistry, vol. 20, 1981, pps. 3615-3621.
Mikhailopulo, I.A. et al.; “1-deaza and 3-deazapurines in the reaction of microbiological transglycosylation”, Biotechnology Letters, vol. 14, No. 10, Oct. 1992, pps. 885-890.
Krenitsky, T.A. et al.; “An enzymic synthesis of purine D-arabinonucleosides”, Carbohydrate Research, vol. 97, 1981, pps. 139-146.
Mikhailopuolo, A.I. et al.; “Benzimidazoles in the reaction of enzymatic transglycosylation”, Nucleosides & Nucleotides, vol. 14, No. 3-5, 1995, pps. 477-480.
Mayer, M.P.; “A new set of useful cloning and expression vectors derived from pBlueScript”, Gene, NL, Elsevier Biomedical Press Amsterdam, vol. 163, No. 1, Sep. 22, 1995, pps. 41-46.
Zintchenko, A.I. et al.; “Substrate specificity of uridine and purine nucleoside phosphorylases of the wohole cells ofEscherichia coli”, Nucleic Acids Research Symposium Series, vol. 18, 1987, pps. 137-140.
PCT International Search Report (PCT/ISA/210) 9 pages from PCT/EP 99/10416.
PCT Partial International Search Results Communication (PCT/ISA/206) 4 pages from PCT/EP/99/0416.
Bestetti Giuseppina
Cali' Simona
Ghisotti Daniela
Orsini Gaetano
Tonon Giancarlo
Darby & Darby
Keryos SpA
Steadman David J.
LandOfFree
Vectors, host cells, and methods for production of uridine... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Vectors, host cells, and methods for production of uridine..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vectors, host cells, and methods for production of uridine... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3496346