Vectors for use in filamentous fungi

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4352541, 43525411, 4352543, 4352561, 4353201, 4351721, 4351723, 536 231, 536 241, C12N 1563, C12N 1580

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active

055039910

ABSTRACT:
Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.

REFERENCES:
patent: 5198345 (1993-03-01), Gwynne et al.
E. J. Mullaney et al. "Primary structure of the trpC gene . . . " Mol. Gen. Genet (1985), 199, pp. 37-45.
Talmade, K. et al "Construction of Plasmid Vectors with Unique . . . " Gene, 12 (1980) pp. 235-241.
Svensson, B. et al "Characterization of Two Forms of Glucoamylase . . . ", Carlsberg Res. Commun., vol. 47, pp. 55-69 (1982).
Slocombe, P. et al "High-Level Expression of an Interferon a2 . . . ", Proc. Natl Aca. Sci. USA, vol. 79, pp. 5455-5459, Sep. 1982.
Whittle, D. J. et al "Molecular Cloning of a Cellumomonas fimi . . . " Gene, 17 (1982), pp. 139-145.
Ballance, D. J. "Transformation of Aspergillus nidulans by the . . . " Chemical Abstracts, vol. 98, 1983, p. 150.
Von Heijne, "Patterns of Amio Acids near Signal-Sequence Cleavage . . . " J. Biochem. 133, 17-21 (1983).
Yleton, M. M. et al "Transformation of Aspergillus nidulans by using . . . " Natl Acad. of Sci. USA, vol. 81, Mar. 1984, pp. 1470-1474.
Penttila, M. E. et al "Cloning of Aspergillus niger genes in yeast . . . ", Mol Gen Gene (1984) 194:494-499.
Pateman, D. A. et al "Gene for alcohol utilization in the lower . . . " Chemical Abstracts, vol. 100, 1984, p. 108.
Pateman, J. A. "Regulation of Alcohol Dehydrogenase (ADH) and . . . ", 10-Microbial Biochem, vol. 98, 1983, p. 247.
Rothstein, S. J. et al "Secretions of a wheat a-amylase expressed in yeast", 37 Nature 308 (1984) Apr., No. 5960.
Boel, E. et al "Two different types of intervening . . . " Chemical Abstracts, vol. 101 (1984)m p. 158.
Doy, C. H. et al "Genomic clones of Aspergillus nidulans containing . . . ", 3-Biochem Genetics, vol. 103, 1985, p. 17753.
Lockington, R. A. et al "Cloning and characterization of the ethanol . . . ", Gene, 33 (1985) pp. 137-149.
Buxton, F. P. et al "Transformation of Aspergillus niger using the . . . " Gene, 37 (1985), pp. 207-214.
Van dan Hondel, et al, "Development of a system for analysis . . . ", 3-Biochem. Genetics, vol. 104 (1986) #103399.
Rambosck, J. et al "Recombinant DNA in filamentous fungi progress . . . ", CRC Critical Rev. in Biotech, vol. 6, Issue 4 (1987), pp. 357-393.
Van Gorcom, R. F. M. et al "Expression of an Escherichia coli .beta.-galactosidase fusion . . . " Gene 40 (1985), pp. 99-106.
Suggs et al, 1981 PNAS, 78-6613.
Tuito et al, 1982 EMBOJ 1(s). 603.
Tilburn et al, 1983 Gene 26.205.
Mariatis et al, 1982 "Molecular cloning cold spring" Harbor Laboratory.

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