Vectors for transformation by ascomycetes

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4351723, 435254, 435320, 435849, 435913, 536 27, 935 26, 935 29, 935 34, 935 41, 935 68, 935 73, 935 80, C12N 120, C12N 1500, C12N 114, C07H 1512

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048164054

ABSTRACT:
Vectors and procedures are provided that enable genetic manipulation of the filamentous ascomycetes such as Aspergillus nidulans and Aspergillus niger. The systems of the invention permit transformation of various Aspergillus strains as well as the production and secretion of desired foreign proteins. Also provided are cosmid vectors which enable the isolation, cloning, sequencing and modifications of genes from the filamentous ascomycetes.

REFERENCES:
Nunberg et al, 1984, "Molecular Cloning and Characterization of the Glucoamylase Gene of Aspergillus awamori", Mol Cell Biol., v4 2306-2315.
Berse et al, 1983, "Cloning and Characterization of the Ornithine Carbamoyltransferase Gene from Aspergillus nidulans", Gene, v 25, 109.117.
Kinghorn et al, 1982, "Cloning and Expression in E. Coli, K-12 of the Biosynthetic Dehydroquinase Function of the arom Cluster Gene . . .", Mol Gen Gen, v 186, 145-152.
Maniatis et al, 1982, Molecular Cloning a Laboratory Manual Cold Spring Harbor Lab, pp. 45-48.
Yelton et al, 1983, "Developmental Regulation of the Aspergillus nidulans trp C Gene", Proc. Natl. Acad. Sci., v 80, 7576-7580 (Dec. 1983).
Yelton et al, 1984, "Transformation of Aspergillus nidulans, by using a trp C Plasmid", Proc. Natl. Acad Sci., v 81, 1470-1474.
Tilburn et al, 1983, "Transformation by Integration in Aspergillus nidulans" Gene, v 26, 205-221.
Hynes et al, 1983, "Isolation of Genomic Clones Containing the Amds Gene of Aspergillus nidulans, . . . ", Mol Cell Bio, v 3, 1430-1439.
Ballance et al., Biochem. and Biophys. Res. Comm. 112(1):284-289, 1983 (Apr. 15).

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