Vectors for the cloning and expression of heterologous genes in

Details Vectors for the cloning and expression of heterologous genes in Vectors for the cloning and expression of heterologous genes in

1989-11-03

1992-11-24

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

4353201, 4351723, 435 699, 435911, 536 27, C12N 1504, C12N 1581

Patent

active

051660709

ABSTRACT:
Vectors for the cloning of heterologous genes in yeast, which have the characteristic that they contain at least the entire DNA of the pKD1 plasmid (isolated from Kluyveromyces drosophilarum) or a part of the same, as well as a DNA segment bearing any heterologous gene and the sequence necessary for the expression of said gene. The high stability of yeasts transformed by said vectors allows their utilization on an industrial scale in various biotechnological fields.

REFERENCES:
Chen et al, J. Basic Microbiol. 28:4 211-220 (1988) A Gene Cloning System for Kluyveromyces lactis.
Das et al, Current Genetics 6:123-128 (1982) A High Frequency Transformation System for . . . Kluyveromyces.
Sreekrishna et al, Gene 28:73-81 (1984) Transformation of Kluyveromyces lactis with the Kanamycin . . . gene . . . Tn903.
Chen et al, Current Genetics 16:95-98 (1989) The Host Range of the pKD1-derived Plasmids in Yeast.
Das, S et al; Transformation of Kluyveromyces fragilis; J. Bacteriol 158(3) p. 1165 (1984).
Falcone, C et al; Analysis of a 1.6 .mu.m circular plasmid from the yeast Kluveromyces drosophilarum: Structure and Molecular Dimorphism; Plasmid 15 p. 248 (1986).
Yamakawa, M et al; Construction of a plasmid--replicating in both S. cerevisiae and Kluyveromyces lactis; Agric Biol Chem 49(5) p. 1537 (1985).
Laudenbach, D. E. et al; Evidence for two distinct origins of replication in the large endogenous plasmid of Anacystis nidulans R2. Mol Gen Genet 199 p. 300 (1985).
Metz, B. A. et al; Identification of an endogenous plasmid in D. discoideum; EMBO 2(4) p. 515 (1983).
Santamaria R et al; Characterization of an endogenous plasmid and development of cloning vectors and a transforming system in Brevibacterium lactofermentum; J. Gen. Microbiol. 130, p. 2237 (1984).
Gerbaud, C. et al; High frequency of yeast transformation by plasmids carrying part or entire 2-.mu.m yeast plasmid; Gene 5 p. 233 (1979).
EPA 0 095 986, claims and p. 4, line 29-p. 10, line 5.
EPA 0 096 910, p. 8, line 1-p. 10, line 20; p. 19, line 21-p. 20, line 12; claims.
EPA 0 096 430-claims.
Journal of Bacteriology, vol. 145, No. 1, Jan. 1981, pp. 382-390 Gunge et al "Isolation and Characterization of Linear Deoxyribonucleic Acid Plasmids From Kluyveromyces lactis and the Plasmid-Associated Killer Character"; p. 382, col. 2.
Nucleic Acids Research, vol. 14, No. 11, 1986, pp. 4471-4481, Chen et al "Sequence Organization of the Circular Plasmid pKD1 from the Yeast Kluyveromyces drosophilarum" (whole article).
Nucleic Acids Research, vol. 12, No. 19, Oct. 1984, pp. 7581-7597 Hishinuma et al "Cloning and Nucleotide of the Linear DNA Killer Plasmis From Yeast".

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