Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1986-02-11
1987-12-29
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435253, 435320, 935 41, 935 42, 935 29, 935 60, C12P 2100, C12N 1500, C12N 120, C12N 100
Patent
active
047161124
ABSTRACT:
Improved expression vectors for amplifying expression of DNA sequence encoding a desired polypeptide beyond that expected by the vector copy number. The vector contains a DNA sequence within the replicon of the vector encoding RNA I and the primer RNA for initiation of DNA replication and their regulatory regions, said DNA sequence having an AT to GC mutuation at position 3029 in the two strands of pBR322; a promoter of RNA I or primer RNA and a restriction site for insertion of a DNA sequence encoding a desired polypeptide into the vector and operatively linking the DNA sequence to the expression control sequence. The AT to GC mutation causes an increase in the vector copy number and further amplifies the expression of the desired polypeptide operatively linked to the RNA I or primer RNA promoter beyond that expected by the copy number increase.
REFERENCES:
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Biogen N.V.
Haley, Jr. James F.
Loring Denise L.
Maurey Karen
Wiseman Thomas G.
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