Vectors for expression of NGF in eukaryotic cells

Details Vectors for expression of NGF in eukaryotic cells Vectors for expression of NGF in eukaryotic cells

1993-11-24

1995-10-10

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 698, 4353201, 530399, 536 2351, C12P 2106, C12N 1500, C07K 1300, C07H 1700

Patent

active

054570344

ABSTRACT:
This invention relates to a process for obtaining, from transformed cells, the polypeptide called nerve growth factor (.beta.-NGF), and more precisely to the process for obtaining, by recombinant DNA technology using genetic constructions insertible in appropriate eukaryotic cell lines, the biologically active human mature form (.beta.-subunit).

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Meier et al. 1986. Mol. clon. of bovine & chick NGF: delineation of cons. & uncons. domains & relationship to bio. act. . . . EMBOJ5:1489.
Selby et al 1987 Mouse NGF gene: Structure and Expression Mol. Cell. Biol. 7(9):3057-3064.
Ullrich et al 1983. Human .beta.-NGF gene sequence highly homologous to that of mouse. Nature 303:321.
Scott et al. 1983. Isolation and nucleotide seq. of cDNA encoding The precursor of m. NGF. Nature 302:538.
Edwards et al. 1988. Processing & Secretion of NGF: Expr. in Mammalian Cells w/Vaccinia Virus Vector Mol. Cell. Biol 8(6):2456.
G. Zettlmeissl et al Biotechnology, vol. 5, No. 7 (Jul. 1987) pp. 720-725.
N. Israel et al Gene, vol. 51, No. 2-3 (1987) pp. 197-204.
Barinaga 1994 Science 264:772-774.
M. Iwane et al., Biochem. Biophys. Res. Comm. 171:116 (1990).

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