Vectors for conditional gene inactivation

Details Vectors for conditional gene inactivation Vectors for conditional gene inactivation

2001-11-16

2008-11-11

Chen, Shin-Lin (Department: 1632)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C435S320100, C435S455000, C435S463000, C514S04400A, C536S023100, C536S024100

Reexamination Certificate

active

07449179

ABSTRACT:
A method of preparing gene trapping libraries, and gene targeted cells for conditional inactivation of genes, is provided. The invention provides a vector having a mutational element cassette and a gene trap cassette, each cassette having site-specific recombination sequences.

REFERENCES:
patent: 5922927 (1999-07-01), Bujard et al.
patent: 6004750 (1999-12-01), Frank-Kamenetskii et al.
patent: 6436707 (2002-08-01), Zambrowicz et al.
patent: 7074611 (2006-07-01), Chambon et al.
patent: 1383891 (2005-12-01), None
patent: WO-99/50426 (1999-10-01), None
patent: WO-01/29208 (2001-04-01), None
Verma et al., 1997, Nature, vol. 389, pp. 239-242.
Eck et al., 1996, Goodman & Gilman's The Pharmacological Basis of Therapeutics, McGraw-Hill, New York, p. 77-101.
Gorecki, 2001, Expert Opin. Emerging Drugs, 6(2): 187-198.
Araki et al., 1997, Nucleic Acids Research, vol. 25, No. 4, p. 868-872.
Araki et al., 1999, Cellular and Moplecular Biology, vol. 45, No. 5, pp. 737-750.
Albert, Henrik, et al., “Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome”,The Plant Journal, 7 (4), (1995), 649-659.
Araki, H., et al., “Site-specific recombinase, R, encoded by yeast plasmid pSR1”,Journal of Molecular Biology. 225(1), (May 1992),25-37.
Bruning, J. C., et al., “Konditionale Mutagenese—Knockout-mause der Zweiten Generation als Modelle Internistischer Erkrankungen”,Medizinische Klinik, 94, (Oct. 15, 1999), 564-569.
Friedrich, G., et al., “Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice”,Genes&Development. 5(9), (Sep. 1991), 1513-1523.
Golic, K. G., et al., “The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome”,Cell. 59(3), (Nov. 1989), 499-509.
Kano, M., et al., “Cre-IoxP-mediated DNA flip-flop in mammalian cells leadint to alternate expression of retrovirally transduced genes”,Biochemical and Biophysical Research Communications, 248 (3), (1998), 806-811.
Kmita, M., et al., “Targeted inversion of a polar silencer within the HoxD complex re-allocates domains of enhancer sharing”,Nature Genetics. 26(4), (Dec. 2000), 451-454.
Lam, K. P., et al., “Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo”,Proceedings of the National Academy of Sciences of the United States of America. 95(22), (1998), 13171-13175.
Michael, S. K., et al., “Efficient Gene-Specific Expression of Cre Recombinase in the Mouse Embryo by Targeted Insertion of a Novel IRES-Cre Cassette into Endogenous Loci”,Mechanisms of Development, 85, (1999), 35-47.
Senecoff, J. F., et al., “DNA recognition by the FLP recombinase of the yeast 2 mu plasmid. A mutational analysis of the FLP binding site”,Journal of Molecular Biology. 201(2), (1988), 405-421.
Wiles, M. V., et al., “Establishment of a gene-trap sequence tag library to generate mutant mice from embryonic stem cells”,Nature Genetics. 24(1), (2000), 13-14.
Zambrowicz, B. P., et al., “Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells”,Nature. 392(6676), (1998), 608-611.

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